The assembly domain of the small capsid protein of Kaposi's sarcoma-associated herpesvirus

Dale Kreitler, Christopher M. Capuano, Brandon W. Henson, Erin N. Pryce, Daniel Anacker, J. Michael McCaffery, Prashant J. Desai

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Self-assembly of Kaposi's sarcoma-associated herpesvirus capsids occurs when six proteins are coexpressed in insect cells using recombinant baculoviruses; however, if the small capsid protein (SCP) is omitted from the coinfection, assembly does not occur. Herein we delineate and identify precisely the assembly domain and the residues of SCP required for assembly. Hence, six residues, R14, D18, V25, R46, G66, and R70 in the assembly domain, when changed to alanine, completely abolish or reduce capsid assembly.

Original languageEnglish (US)
Pages (from-to)11926-11930
Number of pages5
JournalJournal of virology
Volume86
Issue number21
DOIs
StatePublished - Nov 2012

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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