The application of perpendicular and forward light scatter to assess nuclear and cellular morphology

Mitchell C. Benson; Denise C. McDougal; Donald S. Coffey

doi:10.1002/cyto.990050513

The application of perpendicular and forward light scatter to assess nuclear and cellular morphology

Mitchell C. Benson, Denise C. McDougal, Donald S. Coffey

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

Perpendicular and forward light scatter have been employed in a multiparametric approach to distinguish the various lines of the transplantable Dunning R3327 rat prostatic adenocarcinoma. Perpendicular light scatter has been shown to correlate with nuclear size and shape. The average intensity of forward light scatter and perpendicular light scatter signals was demonstrated to increase as cells became less well‐differentiated. The combination of perpendicular and forward light scatter allows for the discrimination of histologically indistinguishable tumors and may therefore be useful to establish a system of grading cancer.

Original language	English (US)
Pages (from-to)	515-522
Number of pages	8
Journal	Cytometry
Volume	5
Issue number	5
DOIs	https://doi.org/10.1002/cyto.990050513
State	Published - Sep 1984
Externally published	Yes

Keywords

Perpendicular light scatter
forward light scatter
nuclear morphology
nuclear roundness
nuclear volume

ASJC Scopus subject areas

Pathology and Forensic Medicine
Biophysics
Hematology
Endocrinology
Cell Biology

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10.1002/cyto.990050513

Cite this

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abstract = "Perpendicular and forward light scatter have been employed in a multiparametric approach to distinguish the various lines of the transplantable Dunning R3327 rat prostatic adenocarcinoma. Perpendicular light scatter has been shown to correlate with nuclear size and shape. The average intensity of forward light scatter and perpendicular light scatter signals was demonstrated to increase as cells became less well‐differentiated. The combination of perpendicular and forward light scatter allows for the discrimination of histologically indistinguishable tumors and may therefore be useful to establish a system of grading cancer.",

keywords = "Perpendicular light scatter, forward light scatter, nuclear morphology, nuclear roundness, nuclear volume",

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year = "1984",

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language = "English (US)",

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AU - Benson, Mitchell C.

AU - McDougal, Denise C.

AU - Coffey, Donald S.

PY - 1984/9

Y1 - 1984/9

N2 - Perpendicular and forward light scatter have been employed in a multiparametric approach to distinguish the various lines of the transplantable Dunning R3327 rat prostatic adenocarcinoma. Perpendicular light scatter has been shown to correlate with nuclear size and shape. The average intensity of forward light scatter and perpendicular light scatter signals was demonstrated to increase as cells became less well‐differentiated. The combination of perpendicular and forward light scatter allows for the discrimination of histologically indistinguishable tumors and may therefore be useful to establish a system of grading cancer.

AB - Perpendicular and forward light scatter have been employed in a multiparametric approach to distinguish the various lines of the transplantable Dunning R3327 rat prostatic adenocarcinoma. Perpendicular light scatter has been shown to correlate with nuclear size and shape. The average intensity of forward light scatter and perpendicular light scatter signals was demonstrated to increase as cells became less well‐differentiated. The combination of perpendicular and forward light scatter allows for the discrimination of histologically indistinguishable tumors and may therefore be useful to establish a system of grading cancer.

KW - Perpendicular light scatter

KW - forward light scatter

KW - nuclear morphology

KW - nuclear roundness

KW - nuclear volume

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VL - 5

SP - 515

EP - 522

JO - Cytometry

JF - Cytometry

IS - 5

ER -

The application of perpendicular and forward light scatter to assess nuclear and cellular morphology

Abstract

Keywords

ASJC Scopus subject areas

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