The application of perpendicular and forward light scatter to assess nuclear and cellular morphology

Mitchell C. Benson, Denise C. McDougal, Donald S. Coffey

Research output: Contribution to journalArticle

Abstract

Perpendicular and forward light scatter have been employed in a multiparametric approach to distinguish the various lines of the transplantable Dunning R3327 rat prostatic adenocarcinoma. Perpendicular light scatter has been shown to correlate with nuclear size and shape. The average intensity of forward light scatter and perpendicular light scatter signals was demonstrated to increase as cells became less well‐differentiated. The combination of perpendicular and forward light scatter allows for the discrimination of histologically indistinguishable tumors and may therefore be useful to establish a system of grading cancer.

Original languageEnglish (US)
Pages (from-to)515-522
Number of pages8
JournalCytometry
Volume5
Issue number5
DOIs
StatePublished - Sep 1984

Keywords

  • forward light scatter
  • nuclear morphology
  • nuclear roundness
  • nuclear volume
  • Perpendicular light scatter

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biophysics
  • Hematology
  • Endocrinology
  • Cell Biology

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