The application of nonlinear Raman spectroscopy to provide molecular specificity in 3-D biological imaging

G. R. Holtom, B. D. Thrall, B. Y. Chin, Terence H Risby

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The molecular specificity in 3-D biological imaging was provided by the application of nonlinear Raman spectroscopy. It had the advantage to obtain selectivity by tuning the difference in laser frequency to match vibrations in molecules of interest. Recording of images was done by raster-scanning the sample through the focal spot. The scan plane has a resolution of about 310 nm.

Original languageEnglish (US)
Title of host publicationConference on Lasers and Electro-Optics Europe - Technical Digest
Pages362-363
Number of pages2
StatePublished - 2001
Externally publishedYes
EventConference on Lasers and Electro-Optics (CLEO) - Baltimore, MD, United States
Duration: May 6 2001May 11 2001

Other

OtherConference on Lasers and Electro-Optics (CLEO)
CountryUnited States
CityBaltimore, MD
Period5/6/015/11/01

Fingerprint

Raman spectroscopy
Tuning
Scanning
Imaging techniques
Molecules
Lasers

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

Cite this

Holtom, G. R., Thrall, B. D., Chin, B. Y., & Risby, T. H. (2001). The application of nonlinear Raman spectroscopy to provide molecular specificity in 3-D biological imaging. In Conference on Lasers and Electro-Optics Europe - Technical Digest (pp. 362-363)

The application of nonlinear Raman spectroscopy to provide molecular specificity in 3-D biological imaging. / Holtom, G. R.; Thrall, B. D.; Chin, B. Y.; Risby, Terence H.

Conference on Lasers and Electro-Optics Europe - Technical Digest. 2001. p. 362-363.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Holtom, GR, Thrall, BD, Chin, BY & Risby, TH 2001, The application of nonlinear Raman spectroscopy to provide molecular specificity in 3-D biological imaging. in Conference on Lasers and Electro-Optics Europe - Technical Digest. pp. 362-363, Conference on Lasers and Electro-Optics (CLEO), Baltimore, MD, United States, 5/6/01.
Holtom GR, Thrall BD, Chin BY, Risby TH. The application of nonlinear Raman spectroscopy to provide molecular specificity in 3-D biological imaging. In Conference on Lasers and Electro-Optics Europe - Technical Digest. 2001. p. 362-363
Holtom, G. R. ; Thrall, B. D. ; Chin, B. Y. ; Risby, Terence H. / The application of nonlinear Raman spectroscopy to provide molecular specificity in 3-D biological imaging. Conference on Lasers and Electro-Optics Europe - Technical Digest. 2001. pp. 362-363
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