A factor chemotactic for eosinophils may be generated by the interaction of a soluble factor derived from antigen-activated lymphocytes with preformed antigen-antibody complexes. The previous demonstration of the in vivo as well as in vitro activity of this factor suggested that it might be of significance in biologic reactions associated with both cell-mediated immunity and antibody formation. Accordingly, guinea pigs were immunized with thyroid extracts in complete Freund's adjuvant to induce thyroiditis, a reaction presumed to be dependent upon cell-mediated immunity for its pathogenesis. Thyroid lesions, consisting of focal lymphoid infiltrates, occurred in the presence or absence of detectable circulating anti-thyroid antibody. With the exception of one guinea pig, eosinophils were found only in the thyroids of those animals with both thyroid lesions and antibody activity. When present, they were located in close proximity to small blood vessels, and in large numbers in the fibrofatty tissue surrounding the gland. Only rarely were they seen in the lymphoid infiltrates themselves. The injection of antibody from these sensitized animals did not lead to the appearance of eosinophils in the thyroids of normal guinea pigs. Thus, in this system, the local accumulation of eosinophils appears to be related to the presence of both lymphocytes in the thyroid and circulating antibody, suggesting that the lymphocyte-derived eosinophil chemotactic factor might be involved in this accumulation.
ASJC Scopus subject areas
- Immunology and Allergy
- Pathology and Forensic Medicine