TY - JOUR
T1 - The antisense transcriptomes of human cells
AU - He, Yiping
AU - Vogelstein, Bert
AU - Velculescu, Victor E.
AU - Papadopoulos, Nickolas
AU - Kinzler, Kenneth W.
PY - 2008/12/19
Y1 - 2008/12/19
N2 - Transcription in mammalian cells can be assessed at a genome-wide level, but it has been difficult to reliably determine whether individual transcripts are derived from the plus or minus strands of chromosomes. This distinction can be critical for understanding the relationship between known transcripts (sense) and the complementary antisense transcripts that may regulate them. Here, we describe a technique that can be used to (i) identify the DNA strand of origin for any particular RNA transcript, and (ii) quantify the number of sense and antisense transcripts from expressed genes at a global level. We examined five different human cell types and in each case found evidence for antisense transcripts in 2900 to 6400 human genes. The distribution of antisense transcripts was distinct from that of sense transcripts, was nonrandom across the genome, and differed among cell types. Antisense transcripts thus appear to be a pervasive feature of human cells, which suggests that they are a fundamental component of gene regulation.
AB - Transcription in mammalian cells can be assessed at a genome-wide level, but it has been difficult to reliably determine whether individual transcripts are derived from the plus or minus strands of chromosomes. This distinction can be critical for understanding the relationship between known transcripts (sense) and the complementary antisense transcripts that may regulate them. Here, we describe a technique that can be used to (i) identify the DNA strand of origin for any particular RNA transcript, and (ii) quantify the number of sense and antisense transcripts from expressed genes at a global level. We examined five different human cell types and in each case found evidence for antisense transcripts in 2900 to 6400 human genes. The distribution of antisense transcripts was distinct from that of sense transcripts, was nonrandom across the genome, and differed among cell types. Antisense transcripts thus appear to be a pervasive feature of human cells, which suggests that they are a fundamental component of gene regulation.
UR - http://www.scopus.com/inward/record.url?scp=57849105533&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=57849105533&partnerID=8YFLogxK
U2 - 10.1126/science.1163853
DO - 10.1126/science.1163853
M3 - Article
C2 - 19056939
AN - SCOPUS:57849105533
SN - 0036-8075
VL - 322
SP - 1855
EP - 1857
JO - Science
JF - Science
IS - 5909
ER -