TY - JOUR
T1 - The anthelmintic drug mebendazole inhibits growth, migration and invasion in gastric cancer cell model
AU - Pinto, Laine Celestino
AU - Soares, Bruno Moreira
AU - Pinheiro, João de Jesus Viana
AU - Riggins, Gregory J.
AU - Assumpção, Paulo Pimentel
AU - Burbano, Rommel Mário Rodriguez
AU - Montenegro, Raquel Carvalho
N1 - Funding Information:
The authors are grateful to the Brazilian Agencies CNPq , CAPES , FAPESPA and Federal University of Para for fellowships and financial support.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - The present study aimed to investigate the effects of MBZ on a human malignant ascites cell line derived from a primary gastric cancer tumor. Our data reveal that MBZ showed high cytotoxicity in vitro, displaying an IC50 of 0.39μM and 1.25μM in ACP-02 and ACP-03, respectively. The association between MBZ and 5-FU increased slightly the cytotoxicity when compared to MBZ and 5-FU alone. Furthermore, MBZ disrupted the microtubule structure of AGP-01 cells and inhibited significantly the invasion and migration of these cells. Activity of active MMP-2 significantly decreased at all tested concentration of MBZ compared to negative control. These results support the indication of MBZ in combination with chemotherapeutic agents as a possible adjuvant therapy for the management/treatment of patients with advanced gastric cancer since MBZ is a drug of low cost with acceptable safety profile and reduced toxicity to normal cells. However, clinical trials must be performed in o to evaluate its efficacy in gastric cancer patients.
AB - The present study aimed to investigate the effects of MBZ on a human malignant ascites cell line derived from a primary gastric cancer tumor. Our data reveal that MBZ showed high cytotoxicity in vitro, displaying an IC50 of 0.39μM and 1.25μM in ACP-02 and ACP-03, respectively. The association between MBZ and 5-FU increased slightly the cytotoxicity when compared to MBZ and 5-FU alone. Furthermore, MBZ disrupted the microtubule structure of AGP-01 cells and inhibited significantly the invasion and migration of these cells. Activity of active MMP-2 significantly decreased at all tested concentration of MBZ compared to negative control. These results support the indication of MBZ in combination with chemotherapeutic agents as a possible adjuvant therapy for the management/treatment of patients with advanced gastric cancer since MBZ is a drug of low cost with acceptable safety profile and reduced toxicity to normal cells. However, clinical trials must be performed in o to evaluate its efficacy in gastric cancer patients.
KW - Invasion
KW - Mebendazole
KW - Metalloproteinase
KW - Migration
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U2 - 10.1016/j.tiv.2015.08.007
DO - 10.1016/j.tiv.2015.08.007
M3 - Article
C2 - 26315676
AN - SCOPUS:84941277289
VL - 29
SP - 2038
EP - 2044
JO - Toxicology in Vitro
JF - Toxicology in Vitro
SN - 0887-2333
IS - 8
ER -