The Anopheles FBN9 immune factor mediates Plasmodium species-specific defense through transgenic fat body expression

Maria L. Simões, Yuemei Dong, Andrew Hammond, Ann Hall, Andrea Crisanti, Tony Nolan, George Dimopoulos

Research output: Research - peer-reviewArticle

Abstract

Mosquitoes have a multifaceted innate immune system that is actively engaged in warding off various pathogens, including the protozoan malaria parasite Plasmodium. Various immune signaling pathways and effectors have been shown to mediate a certain degree of defense specificity against different Plasmodium species. A key pattern recognition receptor of the Anopheles gambiae immune system is the fibrinogen domain-containing immunolectin FBN9, which has been shown to be transcriptonally induced by Plasmodium infection, and to mediate defense against both rodent and human malaria parasites and bacteria. Here we have further studied the defense specificity of FBN9 using a transgenic approach, in which FBN9 is overexpressed in the fat body tissue after a blood meal through a vitellogenin promoter. Interestingly, the Vg-FBN9 transgenic mosquitoes showed increased resistance only to the rodent parasite P. berghei, and not to the human parasite P. falciparum, pointing to differences in the mosquito's defense mechanisms against the two parasite species. The Vg-FBN9 transgenic mosquitoes were also more resistant to infection with both Gram-positive and Gram-negative bacteria and showed increased longevity when infected with P. berghei. Our study points to the importance of both experimentally depleting and enriching candidate anti-Plasmodium effectors in functional studies in order to ascertain their suitability for the development of transgenic mosquito–based malaria control strategies.

LanguageEnglish (US)
Pages257-265
Number of pages9
JournalDevelopmental and Comparative Immunology
Volume67
DOIs
StatePublished - Feb 1 2017

Fingerprint

Anopheles
Fat Body
Plasmodium
Immunologic Factors
Culicidae
Parasites
Malaria
Immune System
Rodentia
Plasmodium malariae
Anopheles gambiae
Vitellogenins
Pattern Recognition Receptors
Gram-Negative Bacteria
Fibrinogen
Meals
Bacteria
Infection

Keywords

  • Anopheles gambiae
  • FBN9
  • Immunity
  • Plasmodium
  • Transgenesis

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

Cite this

The Anopheles FBN9 immune factor mediates Plasmodium species-specific defense through transgenic fat body expression. / Simões, Maria L.; Dong, Yuemei; Hammond, Andrew; Hall, Ann; Crisanti, Andrea; Nolan, Tony; Dimopoulos, George.

In: Developmental and Comparative Immunology, Vol. 67, 01.02.2017, p. 257-265.

Research output: Research - peer-reviewArticle

Simões, Maria L. ; Dong, Yuemei ; Hammond, Andrew ; Hall, Ann ; Crisanti, Andrea ; Nolan, Tony ; Dimopoulos, George. / The Anopheles FBN9 immune factor mediates Plasmodium species-specific defense through transgenic fat body expression. In: Developmental and Comparative Immunology. 2017 ; Vol. 67. pp. 257-265
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