Abstract
A deletion mutant encoding the integrin β3(4I-F56) with an additional Gln at the carboxyl terminus was found occassionally when we were constructing a counterselection yeast two-hybrid assay modle. This mutant exhibited strong transcriptional activation in yeast cells, bearing the Escherichia coli lacZ reporter gene encoding the β-galactosidase under the transcriptional control of GAL4 promoter and TATA box. Further analysis revealed that the region between the amino acid residues 23C to S77, an acidic domain involved in the function of several transcriptional activators were critical for optimal level of transactivation.
Original language | English (US) |
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Pages (from-to) | 1-5 |
Number of pages | 5 |
Journal | Molecular and Cellular Biochemistry |
Volume | 288 |
Issue number | 1-2 |
DOIs | |
State | Published - Aug 2006 |
Externally published | Yes |
Keywords
- Integrin β3
- Transcriptional activator
- Yeast two-hybrid system
ASJC Scopus subject areas
- Molecular Biology
- Clinical Biochemistry
- Cell Biology