The amino-terminal domain of tntegrin β3 functions as a transcriptional activator in yeast

Jianbo Yang; Jia Yao; Lili Chen; Jie Yang

doi:10.1007/s11010-005-9078-2

The amino-terminal domain of tntegrin β3 functions as a transcriptional activator in yeast

Jianbo Yang, Jia Yao, Lili Chen, Jie Yang

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A deletion mutant encoding the integrin β3(4I-F56) with an additional Gln at the carboxyl terminus was found occassionally when we were constructing a counterselection yeast two-hybrid assay modle. This mutant exhibited strong transcriptional activation in yeast cells, bearing the Escherichia coli lacZ reporter gene encoding the β-galactosidase under the transcriptional control of GAL4 promoter and TATA box. Further analysis revealed that the region between the amino acid residues 23C to S77, an acidic domain involved in the function of several transcriptional activators were critical for optimal level of transactivation.

Original language	English (US)
Pages (from-to)	1-5
Number of pages	5
Journal	Molecular and Cellular Biochemistry
Volume	288
Issue number	1-2
DOIs	https://doi.org/10.1007/s11010-005-9078-2
State	Published - Aug 2006
Externally published	Yes

Keywords

Integrin β3
Transcriptional activator
Yeast two-hybrid system

ASJC Scopus subject areas

Molecular Biology
Clinical Biochemistry
Cell Biology

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10.1007/s11010-005-9078-2

Cite this

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title = "The amino-terminal domain of tntegrin β3 functions as a transcriptional activator in yeast",

abstract = "A deletion mutant encoding the integrin β3(4I-F56) with an additional Gln at the carboxyl terminus was found occassionally when we were constructing a counterselection yeast two-hybrid assay modle. This mutant exhibited strong transcriptional activation in yeast cells, bearing the Escherichia coli lacZ reporter gene encoding the β-galactosidase under the transcriptional control of GAL4 promoter and TATA box. Further analysis revealed that the region between the amino acid residues 23C to S77, an acidic domain involved in the function of several transcriptional activators were critical for optimal level of transactivation.",

keywords = "Integrin β3, Transcriptional activator, Yeast two-hybrid system",

author = "Jianbo Yang and Jia Yao and Lili Chen and Jie Yang",

note = "Funding Information: We thank Dr. Peter J. Newman (The Blood Center of South-eastern Wisconsin, Milwaukee, WI, USA) for providing us the recombinant vector pc3a, with integrin β3 cDNA seqence cloned in the vector pcDNA3. This work was financially supported by the Natural Science Foundation of China (Grant No.30171094 and 30271497), also was supported by Foundation of backbone teachers of Education Ministry of China.",

year = "2006",

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doi = "10.1007/s11010-005-9078-2",

language = "English (US)",

volume = "288",

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journal = "Molecular and Cellular Biochemistry",

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T1 - The amino-terminal domain of tntegrin β3 functions as a transcriptional activator in yeast

AU - Yang, Jianbo

AU - Yao, Jia

AU - Chen, Lili

AU - Yang, Jie

N1 - Funding Information: We thank Dr. Peter J. Newman (The Blood Center of South-eastern Wisconsin, Milwaukee, WI, USA) for providing us the recombinant vector pc3a, with integrin β3 cDNA seqence cloned in the vector pcDNA3. This work was financially supported by the Natural Science Foundation of China (Grant No.30171094 and 30271497), also was supported by Foundation of backbone teachers of Education Ministry of China.

PY - 2006/8

Y1 - 2006/8

N2 - A deletion mutant encoding the integrin β3(4I-F56) with an additional Gln at the carboxyl terminus was found occassionally when we were constructing a counterselection yeast two-hybrid assay modle. This mutant exhibited strong transcriptional activation in yeast cells, bearing the Escherichia coli lacZ reporter gene encoding the β-galactosidase under the transcriptional control of GAL4 promoter and TATA box. Further analysis revealed that the region between the amino acid residues 23C to S77, an acidic domain involved in the function of several transcriptional activators were critical for optimal level of transactivation.

AB - A deletion mutant encoding the integrin β3(4I-F56) with an additional Gln at the carboxyl terminus was found occassionally when we were constructing a counterselection yeast two-hybrid assay modle. This mutant exhibited strong transcriptional activation in yeast cells, bearing the Escherichia coli lacZ reporter gene encoding the β-galactosidase under the transcriptional control of GAL4 promoter and TATA box. Further analysis revealed that the region between the amino acid residues 23C to S77, an acidic domain involved in the function of several transcriptional activators were critical for optimal level of transactivation.

KW - Integrin β3

KW - Transcriptional activator

KW - Yeast two-hybrid system

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JO - Molecular and Cellular Biochemistry

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The amino-terminal domain of tntegrin β3 functions as a transcriptional activator in yeast

Abstract

Keywords

ASJC Scopus subject areas

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