The isolated leukocytes donated by 20 highly dust-allergic individuals living in the Baltimore area showed widely different relative sensitivities to dialyzed extracts of the mite Dermatophagoides pteronyssinus (plus growth medium) and a commercial U. S. house dust sample ("U. S. Dust #1"). Most donors, using allergen concentrations determined on a weight basis, were more sensitive to the dust than the mite. There was no significant relationship between sensitivity to dust and mite. Further leukocyte assays with the use of several different dust extracts showed that while there were allergenic differences between extracts, "U. S. Dust #1" was usually the most potent. Immunodiffusion analysis using hyperimmune rabbit anti-mite sera showed that the main antigen shared by the mite and dust extracts was a component of human dander used as part of the mite growth medium. No mite-specific antigens were detectable in the dust extract by this analysis. The anti-mite sera showed little or no ability to block mite-mediated or dust-mediated leukocyte histamine release. For only one donor was blocking of both allergens attained. We conclude that mite components represent only some of the many dust allergens, and that the most potent mite antigens for rabbits are not the major allergens for man.
ASJC Scopus subject areas
- Immunology and Allergy