The adenovirus E4 11k protein binds and relocalizes the cytoplasmic P-body component Ddx6 to aggresomes

Amy E. Greer, Patrick Hearing, Gary Ketner

Research output: Contribution to journalArticle

Abstract

The adenovirus E4 11k protein, product of E4 ORF3, is required in infection for processes including normal accumulation of viral late mRNAs. 11. k restructures both the nucleus and cytoplasm of infected cells by relocalizing specific host cell target proteins, most strikingly components of nuclear PML oncogenic domains. It is likely that in many cases relocalization inactivates target proteins to produce 11. k's effects, although the mechanism and targets for stimulation of late mRNA accumulation is unknown. We have identified a new set of proteins relocalized by 11. k: at least five protein components of cytoplasmic mRNA processing bodies (p-bodies) are found in 11. k-induced cytoplasmic aggresomes, sites where proteins are inactivated or destroyed. One of these p-body proteins, RNA helicase Ddx6, binds 11. k, suggesting a mechanism for relocalization. Because p-bodies are sites for mRNA degradation, their modification by 11. k may provide an explanation for the role of 11. k in viral late mRNA accumulation.

Original languageEnglish (US)
Pages (from-to)161-168
Number of pages8
JournalVirology
Volume417
Issue number1
DOIs
StatePublished - Aug 15 2011

Keywords

  • Adenovirus E4 11k
  • Adenovirus E4 ORF3
  • Aggresome
  • Ddx6
  • Host-virus interaction
  • Late gene expression
  • MRNA degradation
  • P-body
  • RCK
  • Relocalization
  • Reorganization

ASJC Scopus subject areas

  • Virology

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