The active E rosette test: correlation with delayed cutaneous hypersensitivity

P. J. Felsburg, R. Edelman, R. H. Gilman

Research output: Contribution to journalArticle

Abstract

An active subpopulation of peripheral blood T lymphocytes, characterized by rapid (5 min) rosette formation with sheep erythrocytes (A RFC) was measured in normal individuals after they were skin tested with microbial antigens. A significant rise in A RFC occurred in all individuals who developed positive delayed cutaneous hypersensitivity (DCH) reactions, whereas skin test nonresponders showed no significant rise in A RFC. No similar consistent changes occured in populations of total T cells, characterized by longer (60 min) rosette formation with sheep erythrocytes, or in B cells, measured by immunofluorescence of surface immunoglobulin. The A RFC response paralleled the DCH response in timing, but not in intensity. The results provide in vivo evidence for a biologically distinct T cell subpopulation, and focus attention on the A RFC as immunologically active cells.

Original languageEnglish (US)
Pages (from-to)1110-1114
Number of pages5
JournalJ.IMMUNOL.
Volume116
Issue number4
StatePublished - Dec 1 1976
Externally publishedYes

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Delayed Hypersensitivity
Rosette Formation
T-Lymphocytes
Skin
Sheep
Erythrocytes
B-Cell Antigen Receptors
Skin Tests
Fluorescent Antibody Technique
B-Lymphocytes
Antigens
Population

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

The active E rosette test : correlation with delayed cutaneous hypersensitivity. / Felsburg, P. J.; Edelman, R.; Gilman, R. H.

In: J.IMMUNOL., Vol. 116, No. 4, 01.12.1976, p. 1110-1114.

Research output: Contribution to journalArticle

Felsburg, PJ, Edelman, R & Gilman, RH 1976, 'The active E rosette test: correlation with delayed cutaneous hypersensitivity', J.IMMUNOL., vol. 116, no. 4, pp. 1110-1114.
Felsburg, P. J. ; Edelman, R. ; Gilman, R. H. / The active E rosette test : correlation with delayed cutaneous hypersensitivity. In: J.IMMUNOL. 1976 ; Vol. 116, No. 4. pp. 1110-1114.
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