The action of halothane on spontaneous contractile waves and stimulated contractions in isolated rat and dog heart cells

D. M. Wheeler, R. T. Rice, Edward Lakatta

Research output: Contribution to journalArticle

Abstract

The cell length of single, isolated rat and dog heart cells was monitored during exposure to halothane-containing solution to define the cellular mechanism of halothane's negative ionotropic effect. Spontaneous contractile waves, which reflect spontaneous Ca release from the sarcoplasmic reticulum (SR) in resting rat heart cells, exhibited a significant increase in frequency and a decrease in amplitude in the presence of halothane 0.27 mM (0.9 vol%) and 0.55 mM (1.7 vol%). Electrically stimulated dog and rat heart cells abruptly exposed to halothane (0.47-0.55 mM or 1.5-1.7 vol%) revealed a transient increase in twitch amplitude (significantly different from control). Twitch amplitude then declined to values significantly below control as halothane exposure continued. This decrease in twitch reached 42 ± 13% (mean ± SD) of control in rat cells and 50 ± 14% in dog cells beating at 60 beats per min. In dog cells the magnitude of the transient increase in twitch amplitude was greater at faster beating rates compared with lower rates in the same cells (P < 0.01) and the transient increase was insensitive to verapamil. Halothane 0.55 mM (1.7 vol%) also significantly accelerated the rate of decline in the twitch amplitude of successive beats in rat cells stimulated after a rest interval (negative staircase). The findings regarding spontaneous contractile waves indicate a direct effect of halothane at the SR in resting cells, occurring independently of any changes in the slow inward current. The halothane-induced changes in beating cells can be explained by an enhancement of Ca release from the SR with an eventual reduction of SR Ca stores.

Original languageEnglish (US)
Pages (from-to)911-920
Number of pages10
JournalAnesthesiology
Volume72
Issue number5
StatePublished - 1990

Fingerprint

Halothane
Dogs
Sarcoplasmic Reticulum
Verapamil

Keywords

  • Anesthetics, volatile: halothane, negative inotropic effect
  • Heart: contractility; sarcoplasmic reticulum; spontaneous Ca oscillations
  • Ions: calcium

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

The action of halothane on spontaneous contractile waves and stimulated contractions in isolated rat and dog heart cells. / Wheeler, D. M.; Rice, R. T.; Lakatta, Edward.

In: Anesthesiology, Vol. 72, No. 5, 1990, p. 911-920.

Research output: Contribution to journalArticle

@article{781d5b2e79aa48b4885e6005350d297d,
title = "The action of halothane on spontaneous contractile waves and stimulated contractions in isolated rat and dog heart cells",
abstract = "The cell length of single, isolated rat and dog heart cells was monitored during exposure to halothane-containing solution to define the cellular mechanism of halothane's negative ionotropic effect. Spontaneous contractile waves, which reflect spontaneous Ca release from the sarcoplasmic reticulum (SR) in resting rat heart cells, exhibited a significant increase in frequency and a decrease in amplitude in the presence of halothane 0.27 mM (0.9 vol{\%}) and 0.55 mM (1.7 vol{\%}). Electrically stimulated dog and rat heart cells abruptly exposed to halothane (0.47-0.55 mM or 1.5-1.7 vol{\%}) revealed a transient increase in twitch amplitude (significantly different from control). Twitch amplitude then declined to values significantly below control as halothane exposure continued. This decrease in twitch reached 42 ± 13{\%} (mean ± SD) of control in rat cells and 50 ± 14{\%} in dog cells beating at 60 beats per min. In dog cells the magnitude of the transient increase in twitch amplitude was greater at faster beating rates compared with lower rates in the same cells (P < 0.01) and the transient increase was insensitive to verapamil. Halothane 0.55 mM (1.7 vol{\%}) also significantly accelerated the rate of decline in the twitch amplitude of successive beats in rat cells stimulated after a rest interval (negative staircase). The findings regarding spontaneous contractile waves indicate a direct effect of halothane at the SR in resting cells, occurring independently of any changes in the slow inward current. The halothane-induced changes in beating cells can be explained by an enhancement of Ca release from the SR with an eventual reduction of SR Ca stores.",
keywords = "Anesthetics, volatile: halothane, negative inotropic effect, Heart: contractility; sarcoplasmic reticulum; spontaneous Ca oscillations, Ions: calcium",
author = "Wheeler, {D. M.} and Rice, {R. T.} and Edward Lakatta",
year = "1990",
language = "English (US)",
volume = "72",
pages = "911--920",
journal = "Anesthesiology",
issn = "0003-3022",
publisher = "Lippincott Williams and Wilkins",
number = "5",

}

TY - JOUR

T1 - The action of halothane on spontaneous contractile waves and stimulated contractions in isolated rat and dog heart cells

AU - Wheeler, D. M.

AU - Rice, R. T.

AU - Lakatta, Edward

PY - 1990

Y1 - 1990

N2 - The cell length of single, isolated rat and dog heart cells was monitored during exposure to halothane-containing solution to define the cellular mechanism of halothane's negative ionotropic effect. Spontaneous contractile waves, which reflect spontaneous Ca release from the sarcoplasmic reticulum (SR) in resting rat heart cells, exhibited a significant increase in frequency and a decrease in amplitude in the presence of halothane 0.27 mM (0.9 vol%) and 0.55 mM (1.7 vol%). Electrically stimulated dog and rat heart cells abruptly exposed to halothane (0.47-0.55 mM or 1.5-1.7 vol%) revealed a transient increase in twitch amplitude (significantly different from control). Twitch amplitude then declined to values significantly below control as halothane exposure continued. This decrease in twitch reached 42 ± 13% (mean ± SD) of control in rat cells and 50 ± 14% in dog cells beating at 60 beats per min. In dog cells the magnitude of the transient increase in twitch amplitude was greater at faster beating rates compared with lower rates in the same cells (P < 0.01) and the transient increase was insensitive to verapamil. Halothane 0.55 mM (1.7 vol%) also significantly accelerated the rate of decline in the twitch amplitude of successive beats in rat cells stimulated after a rest interval (negative staircase). The findings regarding spontaneous contractile waves indicate a direct effect of halothane at the SR in resting cells, occurring independently of any changes in the slow inward current. The halothane-induced changes in beating cells can be explained by an enhancement of Ca release from the SR with an eventual reduction of SR Ca stores.

AB - The cell length of single, isolated rat and dog heart cells was monitored during exposure to halothane-containing solution to define the cellular mechanism of halothane's negative ionotropic effect. Spontaneous contractile waves, which reflect spontaneous Ca release from the sarcoplasmic reticulum (SR) in resting rat heart cells, exhibited a significant increase in frequency and a decrease in amplitude in the presence of halothane 0.27 mM (0.9 vol%) and 0.55 mM (1.7 vol%). Electrically stimulated dog and rat heart cells abruptly exposed to halothane (0.47-0.55 mM or 1.5-1.7 vol%) revealed a transient increase in twitch amplitude (significantly different from control). Twitch amplitude then declined to values significantly below control as halothane exposure continued. This decrease in twitch reached 42 ± 13% (mean ± SD) of control in rat cells and 50 ± 14% in dog cells beating at 60 beats per min. In dog cells the magnitude of the transient increase in twitch amplitude was greater at faster beating rates compared with lower rates in the same cells (P < 0.01) and the transient increase was insensitive to verapamil. Halothane 0.55 mM (1.7 vol%) also significantly accelerated the rate of decline in the twitch amplitude of successive beats in rat cells stimulated after a rest interval (negative staircase). The findings regarding spontaneous contractile waves indicate a direct effect of halothane at the SR in resting cells, occurring independently of any changes in the slow inward current. The halothane-induced changes in beating cells can be explained by an enhancement of Ca release from the SR with an eventual reduction of SR Ca stores.

KW - Anesthetics, volatile: halothane, negative inotropic effect

KW - Heart: contractility; sarcoplasmic reticulum; spontaneous Ca oscillations

KW - Ions: calcium

UR - http://www.scopus.com/inward/record.url?scp=0025305566&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025305566&partnerID=8YFLogxK

M3 - Article

C2 - 2339805

AN - SCOPUS:0025305566

VL - 72

SP - 911

EP - 920

JO - Anesthesiology

JF - Anesthesiology

SN - 0003-3022

IS - 5

ER -