The 5′-untranslated region of the IGF-I receptor gene modulates reporter gene expression by both pre- and post-transcriptional mechanisms

David W. Cooke, Samuel J. Casella

Research output: Contribution to journalArticle

Abstract

The insulin-like growth factor I (IGF-I) receptor gene has a large, complex 5′-untranslated region (UTR). In order to examine the role that this region plays in regulating IGF-I receptor expression, we isolated fragments of the human IGF-I receptor 5′-UTR and interposed them between the SV40 early promoter and the chloramphenicol acetyl transferase reporter gene. Fragments of the IGF-I receptor gene 5′-UTR were found to enhance reporter gene expression by increasing gene transcription. In addition, the increased transcription and mRNA levels were in excess of the increase in enzyme activity, providing indirect evidence that these fragments inhibit translation, consistent with predictions. Key words: Insulin-like growth factor; Receptor; Insulin-like growth factor receptor; 5′-Untranslated region; Gene expression.

Original languageEnglish (US)
Pages (from-to)77-84
Number of pages8
JournalMolecular and Cellular Endocrinology
Volume101
Issue number1-2
DOIs
StatePublished - May 1994

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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