Abstract
Leukotrienes (LTs), the 5-lipoxygenase (5-LOX) metabolites of arachidonic acid, have roles in many biological processes relevant to the gastrointestinal tract, including intestinal inflammation. We screened two well-known human intestinal epithelial cell lines, HT29 and Caco-2, for evidence of LT-associated enzyme transcripts and LT synthesis. Northern blot analysis of total RNA from both intestinal lines demonstrated high levels of transcripts for LTA4 hydrolase, a multisubstrate enzyme that converts the 5-LOX metabolite, LTA4, to LTB4. With total RNA, the 5-LOX transcript was detected only in HT29. Caco-2 failed to show 5-LOX message even with poly A-containing RNA, although the transcript could be amplified with the polymerase chain reaction. Messenger RNA for FLAP, the 5-lipoxygenase-activating protein, was detectable in both cell lines, but only with poly A-containing RNA. In a sonicated cell preparation, HT29, but not Caco-2, revealed detectable levels of 5-HETE and LTB4. These results suggest that certain intestinal epithelial cells possess a limited capacity to synthesize LTs.
Original language | English (US) |
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Pages (from-to) | 155-166 |
Number of pages | 12 |
Journal | Prostaglandins |
Volume | 49 |
Issue number | 3 |
DOIs | |
State | Published - Mar 1995 |
Keywords
- 5-lipoxygenase
- 5-lipoxygenase-activating protein
- Arachidonic acid
- Caco-2
- HT29
- leukotriene
- leukotriene A hydrolase
ASJC Scopus subject areas
- Biochemistry
- Endocrinology