The 2μm-plasmid-encoded Rep1 and Rep2 proteins interact with each other and colocalize to the Saccharomyces cerevisiae nucleus

Yong Tae Ahn, Xu Li Wu, Shyam Biswal, Soundarapandian Velmurugan, Frederic C. Volkert, Makkuni Jayaram

Research output: Contribution to journalArticle

Abstract

The efficient partitioning of the 2μm plasmid of Saccharomyces cerevisiae at cell division requires two plasmid-encoded proteins (Rep1p and Rep2p) and a cis-acting locus, REP3 (STB). By using protein hybrids containing fusions of the Rep proteins to green fluorescent protein (GFP), we show here that fluorescence from GFP-Rep1p or GFP-Rep2p is almost exclusively localized in the nucleus in a cir+ strain. Nuclear localization of GFP- Rep1p and GFP-Rep2p, though discernible, is less efficient in a cir°host. GFP-Rep2p or GFP-Rep1p is able to promote the stability of a 2μm circle- derived plasmid harboring REP1 or REP2, respectively, in a cir°background. Under these conditions, fluorescence from GFP-Rep2p or GFP-Rep1p is concentrated within the nucleus, as is the case in cir+ cells. This characteristic nuclear accumulation is not dependent on the expression of the FLP or RAF1 gene of the 2μm circle. Nuclear colocalization of Rep1p and Rep2p is consistent with the hypothesis that the two proteins directly or indirectly interact to form a functional bipartite or high-order protein complex. Immunoprecipitation experiments as well as baiting assays using GST- Rep hybrid proteins suggest a direct interaction between Rep1p and Rep2p which, in principle, may be modulated by other yeast proteins. Furthermore, these assays provide evidence for Rep1p-Rep1p and Rep2p-Rep2p associations as well. The sum of these interactions may be important in controlling the effective cellular concentration of the Rep1p-Rep2p complex.

Original languageEnglish (US)
Pages (from-to)7497-7506
Number of pages10
JournalJournal of Bacteriology
Volume179
Issue number23
StatePublished - Dec 1997
Externally publishedYes

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Green Fluorescent Proteins
Saccharomyces cerevisiae
Plasmids
Proteins
Fluorescence
Fungal Proteins
Immunoprecipitation
Cell Division

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

The 2μm-plasmid-encoded Rep1 and Rep2 proteins interact with each other and colocalize to the Saccharomyces cerevisiae nucleus. / Ahn, Yong Tae; Wu, Xu Li; Biswal, Shyam; Velmurugan, Soundarapandian; Volkert, Frederic C.; Jayaram, Makkuni.

In: Journal of Bacteriology, Vol. 179, No. 23, 12.1997, p. 7497-7506.

Research output: Contribution to journalArticle

Ahn, Yong Tae ; Wu, Xu Li ; Biswal, Shyam ; Velmurugan, Soundarapandian ; Volkert, Frederic C. ; Jayaram, Makkuni. / The 2μm-plasmid-encoded Rep1 and Rep2 proteins interact with each other and colocalize to the Saccharomyces cerevisiae nucleus. In: Journal of Bacteriology. 1997 ; Vol. 179, No. 23. pp. 7497-7506.
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abstract = "The efficient partitioning of the 2μm plasmid of Saccharomyces cerevisiae at cell division requires two plasmid-encoded proteins (Rep1p and Rep2p) and a cis-acting locus, REP3 (STB). By using protein hybrids containing fusions of the Rep proteins to green fluorescent protein (GFP), we show here that fluorescence from GFP-Rep1p or GFP-Rep2p is almost exclusively localized in the nucleus in a cir+ strain. Nuclear localization of GFP- Rep1p and GFP-Rep2p, though discernible, is less efficient in a cir°host. GFP-Rep2p or GFP-Rep1p is able to promote the stability of a 2μm circle- derived plasmid harboring REP1 or REP2, respectively, in a cir°background. Under these conditions, fluorescence from GFP-Rep2p or GFP-Rep1p is concentrated within the nucleus, as is the case in cir+ cells. This characteristic nuclear accumulation is not dependent on the expression of the FLP or RAF1 gene of the 2μm circle. Nuclear colocalization of Rep1p and Rep2p is consistent with the hypothesis that the two proteins directly or indirectly interact to form a functional bipartite or high-order protein complex. Immunoprecipitation experiments as well as baiting assays using GST- Rep hybrid proteins suggest a direct interaction between Rep1p and Rep2p which, in principle, may be modulated by other yeast proteins. Furthermore, these assays provide evidence for Rep1p-Rep1p and Rep2p-Rep2p associations as well. The sum of these interactions may be important in controlling the effective cellular concentration of the Rep1p-Rep2p complex.",
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