The 18 kDa isoform of basic fibroblast growth factor is sufficient to stimulate human melanoma growth and angiogenesis

Dennis Fontijn, Monique C A Duyndam, Jeroen A M Beliën, Marielle I. Gallegoz Ruiz, Herbert M. Pinedo, Epie Boven

Research output: Contribution to journalArticlepeer-review

Abstract

Basic fibroblast growth factor is the best-characterized autocrine growth factor in melanoma development and progression. We hypothesized that basic fibroblast growth factor might induce a more aggressive phenotype dependent on the amount of protein expressed in melanoma. Two human melanoma cell lines, M14 and 1F6, known to have low endogenous basic fibroblast growth factor expression and slow growth as subcutaneous xenografts, were stably transfected with vectors encoding either the 18 kDa or all (ALL) isoform proteins of human basic fibroblast growth factor. Different clones overexpressing the 18 kDa or ALL basic fibroblast growth factor proteins were easily obtained. Increased levels of basic fibroblast growth factor were secreted in conditioned medium and stored on the extracellular membrane. Biological activity of the overexpressed basic fibroblast growth factor was confirmed in a human umbilical vein endothelial cell proliferation assay. In 1F6 cells, overexpression of either 18 kDa or ALL basic fibroblast growth factor proteins resulted in up to two-fold shorter in-vitro doubling times (P

Original languageEnglish (US)
Pages (from-to)155-168
Number of pages14
JournalMelanoma Research
Volume17
Issue number3
DOIs
StatePublished - Jun 2007
Externally publishedYes

Keywords

  • Angiogenesis
  • Basic fibroblast growth factor proteins
  • Cutaneous melanoma

ASJC Scopus subject areas

  • Cancer Research
  • Dermatology

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