The 14.6 kd rubber elongation factor (Hev b 1) and 24 kd (Hev b 3) rubber particle proteins are recognized by IgE from patients with spina bifida and latex allergy

Hoong Yeet Yeang, Kay Fong Cheong, Elumalai Sunderasan, Samsidar Hamzah, Nyu Ping Chew, Sharifah Hamid, Robert G. Hamilton, Mary Jane Cardosa

Research output: Contribution to journalArticle

Abstract

Two major water-insoluble proteins are located on the surface of rubber particles in Hevea brasiliensis latex. A 14.6 kd protein (Hev b 1), found mainly on large rubber particles (>350 nm in diameter), and a 24 kd protein (Hev b 3), found mainly on small rubber particles (average diameter, 70 nm), are recognized by IgE from patients with spina bifida and latex allergy. Although Hev b 1 (also called the rubber elongation factor [REF]) has previously been reported as a major latex allergen, this conclusion has been disputed on the basis of results from other studies. The allergenicity of Hev b 1 is verified in this study by testing the recombinant protein generated from its gene. Because allergenicity is confined to patients with spina bifida and not observed in adults sensitive to latex, it is not a major latex allergen. The identification of Hev b 3 as another allergen originating from rubber particles is confirmed by immunogold labeling and electron microscopy. Observations with the monoclonal antibody USM/RC2 developed against Hev b 3 show that the protein has a tendency to fragment into several polypeptides of lower molecular weight (from 24 kd to about 5 kd) when stored at -20°C. There is also indication of protein aggregation from the appearance of proteins with molecular weights greater than 24 kd. Fragmentation of Hev b 3 is induced immediately on the addition of latex B-serum, which is normally compartmentalized in the lutoids in fresh latex. In the preparation of ammoniated latex (used for the manufacture of latex products), the lutoids are ruptured, and the released B-serum reacts with Hev b 3 on the rubber particles to give rise to an array of low molecular weight polypeptides that are allergenic to patients with spina bifida.

Original languageEnglish (US)
Pages (from-to)628-639
Number of pages12
JournalJournal of Allergy and Clinical Immunology
Volume98
Issue number3
DOIs
StatePublished - Jan 1 1996

Keywords

  • Hev b 1
  • Hev b 3
  • Latex
  • USM/RC2
  • immunogold labeling
  • latex allergy
  • monoclonal antibodies
  • rubber particle
  • rubber particle protein
  • spina bifida

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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