TGF-β1 enhances cardiomyogenic differentiation of skeletal muscle-derived adult primitive cells

Ahmed Abdel-Latif, Ewa K. Zuba-Surma, Jamie Case, Sumit Tiwari, Greg Hunt, Smita Ranjan, Robert J. Vincent, Edward F. Srour, Roberto Bolli, Buddhadeb Dawn

Research output: Contribution to journalArticle

Abstract

The optimal medium for cardiac differentiation of adult primitive cells remains to be established. We quantitatively compared the efficacy of IGF-1, dynorphin B, insulin, oxytocin, bFGF, and TGF-β1 in inducing cardiomyogenic differentiation. Adult mouse skeletal muscle-derived Sca1+/CD45-/c-kit-/Thy-1+ (SM+) and Sca1-/CD45-/c-kit-/Thy-1+ (SM-) cells were cultured in basic medium (BM; DMEM, FBS, IGF-1, dynorphin B) alone and BM supplemented with insulin, oxytocin, bFGF, or TGF-β1. Cardiac differentiation was evaluated by the expression of cardiac-specific markers at the mRNA (qRT-PCR) and protein (immunocytochemistry) levels. BM+TGF-β1 upregulated mRNA expression of Nkx2.5 and GATA-4 after 4 days and Myl2 after 9 days. After 30 days, BM+TGF-β1 induced the greatest extent of cardiac differentiation (by morphology and expression of cardiac markers) in SM- cells. We conclude that TGF-β1 enhances cardiomyogenic differentiation in skeletal muscle-derived adult primitive cells. This strategy may be utilized to induce cardiac differentiation as well as to examine the cardiomyogenic potential of adult tissue-derived stem/progenitor cells.

Original languageEnglish (US)
Pages (from-to)514-524
Number of pages11
JournalBasic Research in Cardiology
Volume103
Issue number6
DOIs
StatePublished - 2008
Externally publishedYes

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Skeletal Muscle
Oxytocin
Insulin-Like Growth Factor I
Stem Cells
Messenger RNA
Cultured Cells
Immunohistochemistry
Insulin
Polymerase Chain Reaction
Proteins
SM 1
rimorphin
B-insulin

Keywords

  • Adult stem cell
  • Cardiac differentiation
  • Culture medium
  • Growth factor
  • Skeletal muscle

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)
  • Physiology

Cite this

Abdel-Latif, A., Zuba-Surma, E. K., Case, J., Tiwari, S., Hunt, G., Ranjan, S., ... Dawn, B. (2008). TGF-β1 enhances cardiomyogenic differentiation of skeletal muscle-derived adult primitive cells. Basic Research in Cardiology, 103(6), 514-524. https://doi.org/10.1007/s00395-008-0729-9

TGF-β1 enhances cardiomyogenic differentiation of skeletal muscle-derived adult primitive cells. / Abdel-Latif, Ahmed; Zuba-Surma, Ewa K.; Case, Jamie; Tiwari, Sumit; Hunt, Greg; Ranjan, Smita; Vincent, Robert J.; Srour, Edward F.; Bolli, Roberto; Dawn, Buddhadeb.

In: Basic Research in Cardiology, Vol. 103, No. 6, 2008, p. 514-524.

Research output: Contribution to journalArticle

Abdel-Latif, A, Zuba-Surma, EK, Case, J, Tiwari, S, Hunt, G, Ranjan, S, Vincent, RJ, Srour, EF, Bolli, R & Dawn, B 2008, 'TGF-β1 enhances cardiomyogenic differentiation of skeletal muscle-derived adult primitive cells', Basic Research in Cardiology, vol. 103, no. 6, pp. 514-524. https://doi.org/10.1007/s00395-008-0729-9
Abdel-Latif, Ahmed ; Zuba-Surma, Ewa K. ; Case, Jamie ; Tiwari, Sumit ; Hunt, Greg ; Ranjan, Smita ; Vincent, Robert J. ; Srour, Edward F. ; Bolli, Roberto ; Dawn, Buddhadeb. / TGF-β1 enhances cardiomyogenic differentiation of skeletal muscle-derived adult primitive cells. In: Basic Research in Cardiology. 2008 ; Vol. 103, No. 6. pp. 514-524.
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