TGF-α regulates TLR expression and function on epidermal keratinocytes

Lloyd S. Miller; Ole E. Sørensen; Philip T. Liu; H. Ray Jalian; Deborah Eshtiaghpour; Behnaz E. Behmanesh; Woosin Chung; Timothy D. Starner; Jenny Kim; Peter A. Sieling; Tomas Ganz; Robert L. Modlin

doi:10.4049/jimmunol.174.10.6137

TGF-α regulates TLR expression and function on epidermal keratinocytes

Lloyd S. Miller, Ole E. Sørensen, Philip T. Liu, H. Ray Jalian, Deborah Eshtiaghpour, Behnaz E. Behmanesh, Woosin Chung, Timothy D. Starner, Jenny Kim, Peter A. Sieling, Tomas Ganz, Robert L. Modlin

Research output: Contribution to journal › Article › peer-review

130 Scopus citations

Abstract

The expression of TLRs on epithelial cells provides a first line of defense against invading pathogens. We investigated the regulated expression and function of TLR5 and TLR9 on human keratinocytes, because we found by immunohistochemistry that these TLRs are expressed in distinct layers of the epidermis. We found that TGF-α, a growth and differentiation factor that is present during wound healing and in psoriasis, increased the expression of both TLR5 and TLR9 on keratinocytes. In addition, TGF-α regulated the function of TLR5 and TLR9, because activation with their respective ligands enhanced the production of IL-8 and human β-defensins. These findings provide evidence that TGF-α up-regulates TLR expression and function, augmenting host defense mechanisms at epithelial surfaces.

Original language	English (US)
Pages (from-to)	6137-6143
Number of pages	7
Journal	Journal of Immunology
Volume	174
Issue number	10
DOIs	https://doi.org/10.4049/jimmunol.174.10.6137
State	Published - May 15 2005
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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title = "TGF-α regulates TLR expression and function on epidermal keratinocytes",

abstract = "The expression of TLRs on epithelial cells provides a first line of defense against invading pathogens. We investigated the regulated expression and function of TLR5 and TLR9 on human keratinocytes, because we found by immunohistochemistry that these TLRs are expressed in distinct layers of the epidermis. We found that TGF-α, a growth and differentiation factor that is present during wound healing and in psoriasis, increased the expression of both TLR5 and TLR9 on keratinocytes. In addition, TGF-α regulated the function of TLR5 and TLR9, because activation with their respective ligands enhanced the production of IL-8 and human β-defensins. These findings provide evidence that TGF-α up-regulates TLR expression and function, augmenting host defense mechanisms at epithelial surfaces.",

author = "Miller, {Lloyd S.} and S{\o}rensen, {Ole E.} and Liu, {Philip T.} and Jalian, {H. Ray} and Deborah Eshtiaghpour and Behmanesh, {Behnaz E.} and Woosin Chung and Starner, {Timothy D.} and Jenny Kim and Sieling, {Peter A.} and Tomas Ganz and Modlin, {Robert L.}",

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T1 - TGF-α regulates TLR expression and function on epidermal keratinocytes

AU - Miller, Lloyd S.

AU - Sørensen, Ole E.

AU - Liu, Philip T.

AU - Jalian, H. Ray

AU - Eshtiaghpour, Deborah

AU - Behmanesh, Behnaz E.

AU - Chung, Woosin

AU - Starner, Timothy D.

AU - Kim, Jenny

AU - Sieling, Peter A.

AU - Ganz, Tomas

AU - Modlin, Robert L.

PY - 2005/5/15

Y1 - 2005/5/15

N2 - The expression of TLRs on epithelial cells provides a first line of defense against invading pathogens. We investigated the regulated expression and function of TLR5 and TLR9 on human keratinocytes, because we found by immunohistochemistry that these TLRs are expressed in distinct layers of the epidermis. We found that TGF-α, a growth and differentiation factor that is present during wound healing and in psoriasis, increased the expression of both TLR5 and TLR9 on keratinocytes. In addition, TGF-α regulated the function of TLR5 and TLR9, because activation with their respective ligands enhanced the production of IL-8 and human β-defensins. These findings provide evidence that TGF-α up-regulates TLR expression and function, augmenting host defense mechanisms at epithelial surfaces.

AB - The expression of TLRs on epithelial cells provides a first line of defense against invading pathogens. We investigated the regulated expression and function of TLR5 and TLR9 on human keratinocytes, because we found by immunohistochemistry that these TLRs are expressed in distinct layers of the epidermis. We found that TGF-α, a growth and differentiation factor that is present during wound healing and in psoriasis, increased the expression of both TLR5 and TLR9 on keratinocytes. In addition, TGF-α regulated the function of TLR5 and TLR9, because activation with their respective ligands enhanced the production of IL-8 and human β-defensins. These findings provide evidence that TGF-α up-regulates TLR expression and function, augmenting host defense mechanisms at epithelial surfaces.

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TGF-α regulates TLR expression and function on epidermal keratinocytes

Abstract

ASJC Scopus subject areas

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