TFIIIB subunit Bdp1p is required for periodic integration of the Ty1 retrotransposon and targeting of Isw2p to S. cerevisiae tDNAs

Nurjana Bachman, Marnie E. Gelbart, Toshio Tsukiyama, Jef D. Boeke

Research output: Contribution to journalArticle

Abstract

Retrotransposons are RNA elements that reverse transcribe their RNA genomes and make a cDNA copy that is inserted back into a new genomic location by the element-encoded integrase protein. Ty1 is a long terminal repeat (LTR) retrotransposon in Saccharomyces cerevisiae that inserts into an ∼700-bp integration window upstream of tRNA genes with a periodicity of ∼80 bp. ATP-dependent chromatin remodeling by Isw2 upstream of tRNA genes leads to changes in chromatin structure and Ty1 integration site selection. We show that the N terminus of Bdp1p, a component of the RNA polymerase III transcription factor TFIIIB, is required for periodic integration of Ty1 into the integration window. Deletion of the Bdp1p N terminus and mutation of ISW2 result in similar disruption of nucleosome positioning upstream of some tRNA genes, and the N-terminal domain of Bdp1p is required for targeting of Isw2 complex to tRNA genes. This study provides the first example for recruitment of an ATP-dependent chromatin-remodeling factor by a general transcription factor in vivo.

Original languageEnglish (US)
Pages (from-to)955-964
Number of pages10
JournalGenes & development
Volume19
Issue number8
DOIs
StatePublished - Apr 15 2005

Fingerprint

Transcription Factor TFIIIB
Retroelements
Transfer RNA
Saccharomyces cerevisiae
Chromatin Assembly and Disassembly
Genes
Adenosine Triphosphate
General Transcription Factors
RNA
RNA Polymerase III
Integrases
Terminal Repeat Sequences
Nucleosomes
Periodicity
Chromatin
Complementary DNA
Genome
Mutation
Proteins

Keywords

  • Chromatin
  • Integrase
  • Retrotransposon
  • RNA polymerase III
  • Transcription factor
  • tRNA gene

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

TFIIIB subunit Bdp1p is required for periodic integration of the Ty1 retrotransposon and targeting of Isw2p to S. cerevisiae tDNAs. / Bachman, Nurjana; Gelbart, Marnie E.; Tsukiyama, Toshio; Boeke, Jef D.

In: Genes & development, Vol. 19, No. 8, 15.04.2005, p. 955-964.

Research output: Contribution to journalArticle

Bachman, Nurjana ; Gelbart, Marnie E. ; Tsukiyama, Toshio ; Boeke, Jef D. / TFIIIB subunit Bdp1p is required for periodic integration of the Ty1 retrotransposon and targeting of Isw2p to S. cerevisiae tDNAs. In: Genes & development. 2005 ; Vol. 19, No. 8. pp. 955-964.
@article{59bd9b5127c449b6bca1a9c0e4ebddfc,
title = "TFIIIB subunit Bdp1p is required for periodic integration of the Ty1 retrotransposon and targeting of Isw2p to S. cerevisiae tDNAs",
abstract = "Retrotransposons are RNA elements that reverse transcribe their RNA genomes and make a cDNA copy that is inserted back into a new genomic location by the element-encoded integrase protein. Ty1 is a long terminal repeat (LTR) retrotransposon in Saccharomyces cerevisiae that inserts into an ∼700-bp integration window upstream of tRNA genes with a periodicity of ∼80 bp. ATP-dependent chromatin remodeling by Isw2 upstream of tRNA genes leads to changes in chromatin structure and Ty1 integration site selection. We show that the N terminus of Bdp1p, a component of the RNA polymerase III transcription factor TFIIIB, is required for periodic integration of Ty1 into the integration window. Deletion of the Bdp1p N terminus and mutation of ISW2 result in similar disruption of nucleosome positioning upstream of some tRNA genes, and the N-terminal domain of Bdp1p is required for targeting of Isw2 complex to tRNA genes. This study provides the first example for recruitment of an ATP-dependent chromatin-remodeling factor by a general transcription factor in vivo.",
keywords = "Chromatin, Integrase, Retrotransposon, RNA polymerase III, Transcription factor, tRNA gene",
author = "Nurjana Bachman and Gelbart, {Marnie E.} and Toshio Tsukiyama and Boeke, {Jef D.}",
year = "2005",
month = "4",
day = "15",
doi = "10.1101/gad.1299105",
language = "English (US)",
volume = "19",
pages = "955--964",
journal = "Genes and Development",
issn = "0890-9369",
publisher = "Cold Spring Harbor Laboratory Press",
number = "8",

}

TY - JOUR

T1 - TFIIIB subunit Bdp1p is required for periodic integration of the Ty1 retrotransposon and targeting of Isw2p to S. cerevisiae tDNAs

AU - Bachman, Nurjana

AU - Gelbart, Marnie E.

AU - Tsukiyama, Toshio

AU - Boeke, Jef D.

PY - 2005/4/15

Y1 - 2005/4/15

N2 - Retrotransposons are RNA elements that reverse transcribe their RNA genomes and make a cDNA copy that is inserted back into a new genomic location by the element-encoded integrase protein. Ty1 is a long terminal repeat (LTR) retrotransposon in Saccharomyces cerevisiae that inserts into an ∼700-bp integration window upstream of tRNA genes with a periodicity of ∼80 bp. ATP-dependent chromatin remodeling by Isw2 upstream of tRNA genes leads to changes in chromatin structure and Ty1 integration site selection. We show that the N terminus of Bdp1p, a component of the RNA polymerase III transcription factor TFIIIB, is required for periodic integration of Ty1 into the integration window. Deletion of the Bdp1p N terminus and mutation of ISW2 result in similar disruption of nucleosome positioning upstream of some tRNA genes, and the N-terminal domain of Bdp1p is required for targeting of Isw2 complex to tRNA genes. This study provides the first example for recruitment of an ATP-dependent chromatin-remodeling factor by a general transcription factor in vivo.

AB - Retrotransposons are RNA elements that reverse transcribe their RNA genomes and make a cDNA copy that is inserted back into a new genomic location by the element-encoded integrase protein. Ty1 is a long terminal repeat (LTR) retrotransposon in Saccharomyces cerevisiae that inserts into an ∼700-bp integration window upstream of tRNA genes with a periodicity of ∼80 bp. ATP-dependent chromatin remodeling by Isw2 upstream of tRNA genes leads to changes in chromatin structure and Ty1 integration site selection. We show that the N terminus of Bdp1p, a component of the RNA polymerase III transcription factor TFIIIB, is required for periodic integration of Ty1 into the integration window. Deletion of the Bdp1p N terminus and mutation of ISW2 result in similar disruption of nucleosome positioning upstream of some tRNA genes, and the N-terminal domain of Bdp1p is required for targeting of Isw2 complex to tRNA genes. This study provides the first example for recruitment of an ATP-dependent chromatin-remodeling factor by a general transcription factor in vivo.

KW - Chromatin

KW - Integrase

KW - Retrotransposon

KW - RNA polymerase III

KW - Transcription factor

KW - tRNA gene

UR - http://www.scopus.com/inward/record.url?scp=17444372794&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=17444372794&partnerID=8YFLogxK

U2 - 10.1101/gad.1299105

DO - 10.1101/gad.1299105

M3 - Article

VL - 19

SP - 955

EP - 964

JO - Genes and Development

JF - Genes and Development

SN - 0890-9369

IS - 8

ER -