Tetramer staining for the detection of HLA-specific B cells

Donna P. Lucas, Mary S. Leffell, Andrea A. Zachary

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

HLA-specific B cells can be identified, quantified, and isolated after staining with HLA tetramers. Quantification of these B cells can in turn identify individuals who are sensitized to HLA antigens and the isolation of these cells facilitates a variety of experimental investigations.

Original languageEnglish (US)
Title of host publicationTransplantation Immunology
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages313-318
Number of pages6
ISBN (Print)9781627034920
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1034
ISSN (Print)1064-3745

Keywords

  • B lymphocytes
  • HLA antibodies
  • HLA sensitization
  • HLA specific B Lymphocytes
  • HLA tetramers

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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