Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells

Xuekang Cai, Dan Wang, Yasi Gao, Long Yi, Xing Yang, Zhen Xi

Research output: Contribution to journalArticlepeer-review


We developed a fast strain-promoted azide-alkyne cycloaddition reaction (SPAAC) by tetra-fluorinated aromatic azide with a kinetic constant of 3.60 M−1 s−1, which is among the fastest SPAAC ligations reported so far. We successfully employed the reaction for covalent labelling of proteins with high efficiency and for bioimaging of mitochondria in living cells. The reaction could be a generally useful toolbox for chemical biology and biomaterials.

Original languageEnglish (US)
Pages (from-to)23-26
Number of pages4
JournalRSC Advances
Issue number1
StatePublished - Jan 1 2019
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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