Abstract
Stereological methods were employed to determine volume and surface densities of cytoplasmic organelles in Leydig cells of hamster, rat, rabbit, dog, and guinea pig testes. Contralateral testes were perfused in vitro with maximally stimulating gonadotropin concentration to determine the capacity of these testes to secrete testosterone. Significantly different amounts of testosterone were secreted by in vitro perfused testes of the five species when maximally stimulated with ovine LH. Significant differences also were seen in the volume and surface densities of smooth endoplasmic reticulum, mitochondria, rough endoplasmic reticulum, and lipids in Leydig cell cytoplasm of the five species. Most interestingly, linear positive correlations were seen between testosterone secretion and smooth endoplasmic reticulum volume (r = 0.99) and surface (r = 0.99) densities. Thus, virtually all of the differences in testosterone secretion by maximally stimulated testes of five species could be accounted for by between-species differences in the amount of Leydig cell smooth endoplasmic reticulum.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1867-1874 |
| Number of pages | 8 |
| Journal | Endocrinology |
| Volume | 107 |
| Issue number | 6 |
| State | Published - 1980 |
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ASJC Scopus subject areas
- Endocrinology
- Endocrinology, Diabetes and Metabolism
Cite this
Testosterone secretion by rat, rabbit, guinea pig, dog, and hamster testes perfused in vitro : Correlation with Leydig cell ultrastructure. / Zirkin, Barry R; Ewing, L. L.; Kromann, N.; Cochran, R. C.
In: Endocrinology, Vol. 107, No. 6, 1980, p. 1867-1874.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Testosterone secretion by rat, rabbit, guinea pig, dog, and hamster testes perfused in vitro
T2 - Correlation with Leydig cell ultrastructure
AU - Zirkin, Barry R
AU - Ewing, L. L.
AU - Kromann, N.
AU - Cochran, R. C.
PY - 1980
Y1 - 1980
N2 - Stereological methods were employed to determine volume and surface densities of cytoplasmic organelles in Leydig cells of hamster, rat, rabbit, dog, and guinea pig testes. Contralateral testes were perfused in vitro with maximally stimulating gonadotropin concentration to determine the capacity of these testes to secrete testosterone. Significantly different amounts of testosterone were secreted by in vitro perfused testes of the five species when maximally stimulated with ovine LH. Significant differences also were seen in the volume and surface densities of smooth endoplasmic reticulum, mitochondria, rough endoplasmic reticulum, and lipids in Leydig cell cytoplasm of the five species. Most interestingly, linear positive correlations were seen between testosterone secretion and smooth endoplasmic reticulum volume (r = 0.99) and surface (r = 0.99) densities. Thus, virtually all of the differences in testosterone secretion by maximally stimulated testes of five species could be accounted for by between-species differences in the amount of Leydig cell smooth endoplasmic reticulum.
AB - Stereological methods were employed to determine volume and surface densities of cytoplasmic organelles in Leydig cells of hamster, rat, rabbit, dog, and guinea pig testes. Contralateral testes were perfused in vitro with maximally stimulating gonadotropin concentration to determine the capacity of these testes to secrete testosterone. Significantly different amounts of testosterone were secreted by in vitro perfused testes of the five species when maximally stimulated with ovine LH. Significant differences also were seen in the volume and surface densities of smooth endoplasmic reticulum, mitochondria, rough endoplasmic reticulum, and lipids in Leydig cell cytoplasm of the five species. Most interestingly, linear positive correlations were seen between testosterone secretion and smooth endoplasmic reticulum volume (r = 0.99) and surface (r = 0.99) densities. Thus, virtually all of the differences in testosterone secretion by maximally stimulated testes of five species could be accounted for by between-species differences in the amount of Leydig cell smooth endoplasmic reticulum.
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UR - http://www.scopus.com/inward/citedby.url?scp=0019163930&partnerID=8YFLogxK
M3 - Article
C2 - 7428694
AN - SCOPUS:0019163930
VL - 107
SP - 1867
EP - 1874
JO - Endocrinology
JF - Endocrinology
SN - 0013-7227
IS - 6
ER -