Testing the critical window hypothesis of timing and duration of estradiol treatment on hypothalamic gene networks in reproductively mature and aging female rats

Weiling Yin, Sean M. Maguire, Brian Pham, Alexandra N. Garcia, Nguyen Vy Dang, Jingya Liang, Andrew Wolfe, Hans A. Hofmann, Andrea C. Gore

Research output: Contribution to journalArticle

Abstract

At menopause, the dramatic loss of ovarian estradiol (E2) necessitates the adaptation of estrogensensitive neurons in the hypothalamus to an estrogen-depleted environment. We developed a rat model to test the "critical window" hypothesis of the effects of timing and duration of E2 treatment after deprivation on the hypothalamic neuronal gene network in the arcuate nucleus and the medial preoptic area. Rats at 2 ages (reproductively mature or aging) were ovariectomized and given E2 or vehicle replacement regimes of differing timing and duration. Using a 48-gene quantitative low-density PCR array and weighted gene coexpression network analysis,weidentified gene modules differentially regulated by age, timing, and duration of E2 treatment. Of particular interest, E2 status differentially affected suites of genes in the hypothalamus involved in energy balance, circadian rhythms,andreproduction.In fact,E2statuswasthedominantfactorindetermininggenemodulesand hormone levels; age, timing, and duration had more subtle effects. Our results highlight the plasticity of hypothalamic neuroendocrine systems during reproductive aging and its surprising ability to adapt to diverse E2 replacement regimes.

Original languageEnglish (US)
Pages (from-to)2918-2933
Number of pages16
JournalEndocrinology
Volume156
Issue number8
DOIs
StatePublished - Aug 1 2015

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Gene Regulatory Networks
Estradiol
Hypothalamus
Arcuate Nucleus of Hypothalamus
Neurosecretory Systems
Preoptic Area
Menopause
Circadian Rhythm
Genes
Estrogens
Hormones
Neurons
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Endocrinology

Cite this

Testing the critical window hypothesis of timing and duration of estradiol treatment on hypothalamic gene networks in reproductively mature and aging female rats. / Yin, Weiling; Maguire, Sean M.; Pham, Brian; Garcia, Alexandra N.; Dang, Nguyen Vy; Liang, Jingya; Wolfe, Andrew; Hofmann, Hans A.; Gore, Andrea C.

In: Endocrinology, Vol. 156, No. 8, 01.08.2015, p. 2918-2933.

Research output: Contribution to journalArticle

Yin, Weiling ; Maguire, Sean M. ; Pham, Brian ; Garcia, Alexandra N. ; Dang, Nguyen Vy ; Liang, Jingya ; Wolfe, Andrew ; Hofmann, Hans A. ; Gore, Andrea C. / Testing the critical window hypothesis of timing and duration of estradiol treatment on hypothalamic gene networks in reproductively mature and aging female rats. In: Endocrinology. 2015 ; Vol. 156, No. 8. pp. 2918-2933.
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