Test-retest reliability of pain-related brain activity in healthy controls undergoing experimental thermal pain

Janelle E. Letzen, Landrew S. Sevel, Charles W. Gay, Andrew M. O'Shea, Jason G. Craggs, Donald D. Price, Michael E. Robinson

Research output: Contribution to journalArticlepeer-review


Although functional magnetic resonance imaging (fMRI) has been proposed as a method to elucidate pain-related biomarkers, little information exists related to psychometric properties of fMRI findings. This knowledge is essential for potential translation of this technology to clinical settings. The purpose of this study was to assess the test-retest reliability of pain-related brain activity and how it compares to the reliability of self-report. Twenty-two healthy controls (mean age = 22.6 years, standard deviation = 2.9) underwent 3 runs of an fMRI paradigm that used thermal stimuli to elicit experimental pain. Functional MRI summary statistics related to brain activity during thermal stimulation periods were extracted from bilateral anterior cingulate cortices and anterior insula. Intraclass correlations (ICCs) were conducted on these summary statistics and generally showed "good" test-retest reliability in all regions of interest (ICC range =.32-.88; mean =.71); however, these results did not surpass ICC values from pain ratings, which fell within the "excellent" range (ICC range =.93-.96; mean =.94). Findings suggest that fMRI is a valuable tool for measuring pain mechanisms but did not show an adequate level of test-retest reliability for fMRI to potentially act as a surrogate for individuals' self-report of pain. Perspective This study is one of the first reports to demonstrate the test-retest reliability of fMRI findings related to pain processing and provides a comparison to the reliability of subjective reports of pain. This information is essential for determining whether fMRI technology should be potentially translated for clinical use.

Original languageEnglish (US)
Pages (from-to)1008-1014
Number of pages7
JournalJournal of Pain
Issue number10
StatePublished - Oct 1 2014
Externally publishedYes


  • Functional magnetic resonance imaging
  • anterior cingulate cortex
  • insula
  • test-retest reliability

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Anesthesiology and Pain Medicine


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