Termination of phototransduction requires binding of the NINAC myosin III and the PDZ protein INAD

Paul D. Wes, Xian Zhong Shawn Xu, Hong Sheng Li, Fred Chien, Stephen K. Doberstein, Craig Montell

Research output: Contribution to journalArticle

Abstract

Many of the proteins that are critical for Drosophila phototransduction assemble into a signaling complex, signalplex, through association with the PDZ-domain protein INAD. Some of these proteins depend on INAD for proper subcellular localization to the phototransducing organelle, the rhabdomere, making it difficult to assess any physiological function of this signaling complex independent of localization. Here we demonstrated that INAD bound directly to the NINAC myosin III, yet the subcellular localization of NINAC was normal in inaD mutants. Nevertheless, the INAD binding site was sufficient to target a heterologous protein to the rhabdomeres. Disruption of the NINAC/INAD interaction delayed termination of the photoreceptor response. Thus one role of this signaling complex is in rapid deactivation of the photoresponse.

Original languageEnglish (US)
Pages (from-to)447-453
Number of pages7
JournalNature Neuroscience
Volume2
Issue number5
DOIs
StatePublished - May 1999

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Myosin Type III
Light Signal Transduction
PDZ Domains
Proteins
Organelles
Binding Sites

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Wes, P. D., Xu, X. Z. S., Li, H. S., Chien, F., Doberstein, S. K., & Montell, C. (1999). Termination of phototransduction requires binding of the NINAC myosin III and the PDZ protein INAD. Nature Neuroscience, 2(5), 447-453. https://doi.org/10.1038/8116

Termination of phototransduction requires binding of the NINAC myosin III and the PDZ protein INAD. / Wes, Paul D.; Xu, Xian Zhong Shawn; Li, Hong Sheng; Chien, Fred; Doberstein, Stephen K.; Montell, Craig.

In: Nature Neuroscience, Vol. 2, No. 5, 05.1999, p. 447-453.

Research output: Contribution to journalArticle

Wes, PD, Xu, XZS, Li, HS, Chien, F, Doberstein, SK & Montell, C 1999, 'Termination of phototransduction requires binding of the NINAC myosin III and the PDZ protein INAD', Nature Neuroscience, vol. 2, no. 5, pp. 447-453. https://doi.org/10.1038/8116
Wes, Paul D. ; Xu, Xian Zhong Shawn ; Li, Hong Sheng ; Chien, Fred ; Doberstein, Stephen K. ; Montell, Craig. / Termination of phototransduction requires binding of the NINAC myosin III and the PDZ protein INAD. In: Nature Neuroscience. 1999 ; Vol. 2, No. 5. pp. 447-453.
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