Terminal complement components mediate release of von Willebrand factor and adhesion of platelets in arteries of allografts

Hirofumi Ota, Karen Fox-Talbot, Weimin Hu, Zhiping Qian, Fred Sanfilippo, Ralph H. Hruban, William M. Baldwin

Research output: Contribution to journalArticle

Abstract

Background. Both humoral and cellular immune responses can cause arterial injury in organ transplants, but the manifestations of these different inflammatory mechanisms have not been dissected fully. The present study was designed to define the effects of the terminal complement components on arterial injury in vivo. Methods. The authors have developed congenic rat strains with a C6 deficiency. The absence of C6 terminates the cascade of complement after C5 cleavage and prevents the assembly of the membrane attack complex. Hearts were transplanted from PVG.1A (RT1a) rats to major histocompatibility complex-incompatible C6-deficient (C6-) or C6-sufficient (C6+) PVG.1U (RT1 u) rats. Results. PVG.1A (C6-) cardiac grafts were rejected acutely (6-7 days) by untreated PVG.1U (C6+) recipients but survived significantly longer in PVG.1U (C6-) recipients (8 to > 30 days). Arteries of cardiac allografts in C6+ recipients demonstrated extensive endothelial injury evidenced by release of von Willebrand factor (vWF) and accompanied by platelet aggregation. In contrast, vWF was retained in Weibel-Palade storage granules of arterial endothelial cells in cardiac allografts that were rejected by C6- recipients. In the absence of C6, intimai alterations were limited to lifting of endothelial cells from supporting stroma by infiltrating mononuclear cells, duplicating the clinical lesion described as endotheliitis or intimal arteritis. Delaying graft rejection with a short course of cyclosporine did not decrease vWF release and platelet aggregation in PVG.1U (C6+) recipients. Conclusions. Mononuclear cell infiltration of the arterial intima occurs in the absence of C6, but C6 deficiency limits the release of vWF from arterial endothelial cells.

Original languageEnglish (US)
Pages (from-to)276-281
Number of pages6
JournalTransplantation
Volume79
Issue number3
DOIs
StatePublished - Feb 15 2005

Keywords

  • Antibody
  • Complement
  • Macrophages
  • Platelets
  • Von Willebrand factor

ASJC Scopus subject areas

  • Transplantation

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