Teratospermia in domestic cats compromises penetration of zona-free hamster ova and cat zonae pellucidae

J. Howard, M. Bush, D. E. Wildt

Research output: Contribution to journalArticle

Abstract

The ability of spermatozoa to bind and penetrate zona-free hamster ova and the zonae pellucidae of domestic cat oocytes in vitro was compared between normospermic (>60% structurally normal spermatozoa per ejaculate) and teratospermic ( 0.05) from similarly treated normospermic aliquots. However, the percent penetration of hamster ova by normospermic ejaculates (10.5%) was superior (P <0.05) to that of teratospermic ejaculates (2.8%). Although swim-up processing improved percent sperm motility, progressive motility, and normal morphology in teratospermic ejaculates (P <0.05), no difference was observed in ovum penetration among the DR-treated, NS-treated, and SU-treated spermatozoa (P > 0.05). Culture medium had no effect on sperm binding in the hamster assay, but ovum penetration rate by spermatozoa in the normospermic ejaculates was enhanced (P <0.05) using mKRB (13.5%) when compared with BWW (7.6%) medium. Spermatozoa from teratospermic cats were capable of binding and penetrating cat zonae; however, sperm-zona interaction (defined as percent of oocytes with spermatozoa binding to or penetrating into the zona) was different (P <0.05) between normospermic (65.3%) and teratospermic (24.2%) cats. The number of bound spermatozoa per ovum was five-fold less in males producing high proportions of pleiomorphic spermatozoa (P <0.05). Zona penetration was also different between normospermic (58.1%) and teratospermic (16.9%) males. Within cat populations, the DR, NS, and SU treatments had no influence on sperm binding or zona penetration. These results indicate that teratospermia in domestic cats has a detrimental impact on gamete interaction and penetration of zona-free hamster ova and cat zonae pellucidae. The in vitro effect is not entirely dependent on absolute numbers of structurally normal spermatozoa but appears to be related to an unidentified characteristic of teratospermic ejaculates.

Original languageEnglish (US)
Pages (from-to)36-45
Number of pages10
JournalJournal of Andrology
Volume12
Issue number1
StatePublished - 1991
Externally publishedYes

Fingerprint

Zona Pellucida
Herpes Zoster
Cricetinae
Ovum
Spermatozoa
Cats
Oocytes
Teratozoospermia
Sperm-Ovum Interactions
Culture Media

Keywords

  • Sperm function
  • Sperm morphology
  • Teratospermia
  • Zona pellucida
  • Zona-free hamster ova

ASJC Scopus subject areas

  • Endocrinology

Cite this

Teratospermia in domestic cats compromises penetration of zona-free hamster ova and cat zonae pellucidae. / Howard, J.; Bush, M.; Wildt, D. E.

In: Journal of Andrology, Vol. 12, No. 1, 1991, p. 36-45.

Research output: Contribution to journalArticle

@article{e1c8c06f29364d818a064184adc4f4c6,
title = "Teratospermia in domestic cats compromises penetration of zona-free hamster ova and cat zonae pellucidae",
abstract = "The ability of spermatozoa to bind and penetrate zona-free hamster ova and the zonae pellucidae of domestic cat oocytes in vitro was compared between normospermic (>60{\%} structurally normal spermatozoa per ejaculate) and teratospermic ( 0.05) from similarly treated normospermic aliquots. However, the percent penetration of hamster ova by normospermic ejaculates (10.5{\%}) was superior (P <0.05) to that of teratospermic ejaculates (2.8{\%}). Although swim-up processing improved percent sperm motility, progressive motility, and normal morphology in teratospermic ejaculates (P <0.05), no difference was observed in ovum penetration among the DR-treated, NS-treated, and SU-treated spermatozoa (P > 0.05). Culture medium had no effect on sperm binding in the hamster assay, but ovum penetration rate by spermatozoa in the normospermic ejaculates was enhanced (P <0.05) using mKRB (13.5{\%}) when compared with BWW (7.6{\%}) medium. Spermatozoa from teratospermic cats were capable of binding and penetrating cat zonae; however, sperm-zona interaction (defined as percent of oocytes with spermatozoa binding to or penetrating into the zona) was different (P <0.05) between normospermic (65.3{\%}) and teratospermic (24.2{\%}) cats. The number of bound spermatozoa per ovum was five-fold less in males producing high proportions of pleiomorphic spermatozoa (P <0.05). Zona penetration was also different between normospermic (58.1{\%}) and teratospermic (16.9{\%}) males. Within cat populations, the DR, NS, and SU treatments had no influence on sperm binding or zona penetration. These results indicate that teratospermia in domestic cats has a detrimental impact on gamete interaction and penetration of zona-free hamster ova and cat zonae pellucidae. The in vitro effect is not entirely dependent on absolute numbers of structurally normal spermatozoa but appears to be related to an unidentified characteristic of teratospermic ejaculates.",
keywords = "Sperm function, Sperm morphology, Teratospermia, Zona pellucida, Zona-free hamster ova",
author = "J. Howard and M. Bush and Wildt, {D. E.}",
year = "1991",
language = "English (US)",
volume = "12",
pages = "36--45",
journal = "Journal of Andrology",
issn = "0196-3635",
publisher = "American Society of Andrology Inc.",
number = "1",

}

TY - JOUR

T1 - Teratospermia in domestic cats compromises penetration of zona-free hamster ova and cat zonae pellucidae

AU - Howard, J.

AU - Bush, M.

AU - Wildt, D. E.

PY - 1991

Y1 - 1991

N2 - The ability of spermatozoa to bind and penetrate zona-free hamster ova and the zonae pellucidae of domestic cat oocytes in vitro was compared between normospermic (>60% structurally normal spermatozoa per ejaculate) and teratospermic ( 0.05) from similarly treated normospermic aliquots. However, the percent penetration of hamster ova by normospermic ejaculates (10.5%) was superior (P <0.05) to that of teratospermic ejaculates (2.8%). Although swim-up processing improved percent sperm motility, progressive motility, and normal morphology in teratospermic ejaculates (P <0.05), no difference was observed in ovum penetration among the DR-treated, NS-treated, and SU-treated spermatozoa (P > 0.05). Culture medium had no effect on sperm binding in the hamster assay, but ovum penetration rate by spermatozoa in the normospermic ejaculates was enhanced (P <0.05) using mKRB (13.5%) when compared with BWW (7.6%) medium. Spermatozoa from teratospermic cats were capable of binding and penetrating cat zonae; however, sperm-zona interaction (defined as percent of oocytes with spermatozoa binding to or penetrating into the zona) was different (P <0.05) between normospermic (65.3%) and teratospermic (24.2%) cats. The number of bound spermatozoa per ovum was five-fold less in males producing high proportions of pleiomorphic spermatozoa (P <0.05). Zona penetration was also different between normospermic (58.1%) and teratospermic (16.9%) males. Within cat populations, the DR, NS, and SU treatments had no influence on sperm binding or zona penetration. These results indicate that teratospermia in domestic cats has a detrimental impact on gamete interaction and penetration of zona-free hamster ova and cat zonae pellucidae. The in vitro effect is not entirely dependent on absolute numbers of structurally normal spermatozoa but appears to be related to an unidentified characteristic of teratospermic ejaculates.

AB - The ability of spermatozoa to bind and penetrate zona-free hamster ova and the zonae pellucidae of domestic cat oocytes in vitro was compared between normospermic (>60% structurally normal spermatozoa per ejaculate) and teratospermic ( 0.05) from similarly treated normospermic aliquots. However, the percent penetration of hamster ova by normospermic ejaculates (10.5%) was superior (P <0.05) to that of teratospermic ejaculates (2.8%). Although swim-up processing improved percent sperm motility, progressive motility, and normal morphology in teratospermic ejaculates (P <0.05), no difference was observed in ovum penetration among the DR-treated, NS-treated, and SU-treated spermatozoa (P > 0.05). Culture medium had no effect on sperm binding in the hamster assay, but ovum penetration rate by spermatozoa in the normospermic ejaculates was enhanced (P <0.05) using mKRB (13.5%) when compared with BWW (7.6%) medium. Spermatozoa from teratospermic cats were capable of binding and penetrating cat zonae; however, sperm-zona interaction (defined as percent of oocytes with spermatozoa binding to or penetrating into the zona) was different (P <0.05) between normospermic (65.3%) and teratospermic (24.2%) cats. The number of bound spermatozoa per ovum was five-fold less in males producing high proportions of pleiomorphic spermatozoa (P <0.05). Zona penetration was also different between normospermic (58.1%) and teratospermic (16.9%) males. Within cat populations, the DR, NS, and SU treatments had no influence on sperm binding or zona penetration. These results indicate that teratospermia in domestic cats has a detrimental impact on gamete interaction and penetration of zona-free hamster ova and cat zonae pellucidae. The in vitro effect is not entirely dependent on absolute numbers of structurally normal spermatozoa but appears to be related to an unidentified characteristic of teratospermic ejaculates.

KW - Sperm function

KW - Sperm morphology

KW - Teratospermia

KW - Zona pellucida

KW - Zona-free hamster ova

UR - http://www.scopus.com/inward/record.url?scp=0026022165&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026022165&partnerID=8YFLogxK

M3 - Article

C2 - 2010349

AN - SCOPUS:0026022165

VL - 12

SP - 36

EP - 45

JO - Journal of Andrology

JF - Journal of Andrology

SN - 0196-3635

IS - 1

ER -