Tenascin-C and integrin α9 mediate interactions of prostate cancer with the bone microenvironment

Rebeca San Martin; Ravi Pathak; Antrix Jain; Sung Yun Jung; Susan G. Hilsenbeck; María C. Piña-Barba; Andrew G. Sikora; Kenneth J. Pienta; David R. Rowley

doi:10.1158/0008-5472.CAN-17-0064

Tenascin-C and integrin α9 mediate interactions of prostate cancer with the bone microenvironment

Rebeca San Martin, Ravi Pathak, Antrix Jain, Sung Yun Jung, Susan G. Hilsenbeck, María C. Piña-Barba, Andrew G. Sikora, Kenneth J. Pienta, David R. Rowley

School of Medicine

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Deposition of the extracellular matrix protein tenascin-C is part of the reactive stroma response, which has a critical role in prostate cancer progression. Here, we report that tenascin C is expressed in the bone endosteum and is associated with formation of prostate bone metastases. Metastatic cells cultured on osteo-mimetic surfaces coated with tenascin C exhibited enhanced adhesion and colony formation as mediated by integrin a9b1. In addition, metastatic cells preferentially migrated and colonized tenascin-C–coated trabecular bone xenografts in a novel system that employed chorioallantoic membranes of fertilized chicken eggs as host. Overall, our studies deepen knowledge about reactive stroma responses in the bone endosteum that accompany prostate cancer metastasis to trabecular bone, with potential implications to therapeutically target this process in patients.

Original language	English (US)
Pages (from-to)	5977-5988
Number of pages	12
Journal	Cancer Research
Volume	77
Issue number	21
DOIs	https://doi.org/10.1158/0008-5472.CAN-17-0064
State	Published - Nov 1 2017

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1158/0008-5472.CAN-17-0064

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title = "Tenascin-C and integrin α9 mediate interactions of prostate cancer with the bone microenvironment",

abstract = "Deposition of the extracellular matrix protein tenascin-C is part of the reactive stroma response, which has a critical role in prostate cancer progression. Here, we report that tenascin C is expressed in the bone endosteum and is associated with formation of prostate bone metastases. Metastatic cells cultured on osteo-mimetic surfaces coated with tenascin C exhibited enhanced adhesion and colony formation as mediated by integrin a9b1. In addition, metastatic cells preferentially migrated and colonized tenascin-C–coated trabecular bone xenografts in a novel system that employed chorioallantoic membranes of fertilized chicken eggs as host. Overall, our studies deepen knowledge about reactive stroma responses in the bone endosteum that accompany prostate cancer metastasis to trabecular bone, with potential implications to therapeutically target this process in patients.",

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note = "Funding Information: CPRIT Proteomics and Metabolomics Core Facility Award RP12009, CCSG P30CA125123, and the Comprehensive Cancer Center Grant NIH NCI P30CA125123 (to Baylor College of Medicine). Funding Information: This work is funded by grants from CRPIT RP140616 (to D.R. Rowley), NIH NCI U01CA143055 (to D.R. Rowley and K.J. Pienta), R01CA58093 (to D.R. Rowley), the Caroline Weiss Law Endowment (to A.G. Sikora), the Publisher Copyright: {\textcopyright}2017 AACR.",

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AU - Martin, Rebeca San

AU - Pathak, Ravi

AU - Jain, Antrix

AU - Jung, Sung Yun

AU - Hilsenbeck, Susan G.

AU - Piña-Barba, María C.

AU - Sikora, Andrew G.

AU - Pienta, Kenneth J.

AU - Rowley, David R.

N1 - Funding Information: CPRIT Proteomics and Metabolomics Core Facility Award RP12009, CCSG P30CA125123, and the Comprehensive Cancer Center Grant NIH NCI P30CA125123 (to Baylor College of Medicine). Funding Information: This work is funded by grants from CRPIT RP140616 (to D.R. Rowley), NIH NCI U01CA143055 (to D.R. Rowley and K.J. Pienta), R01CA58093 (to D.R. Rowley), the Caroline Weiss Law Endowment (to A.G. Sikora), the Publisher Copyright: ©2017 AACR.

PY - 2017/11/1

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N2 - Deposition of the extracellular matrix protein tenascin-C is part of the reactive stroma response, which has a critical role in prostate cancer progression. Here, we report that tenascin C is expressed in the bone endosteum and is associated with formation of prostate bone metastases. Metastatic cells cultured on osteo-mimetic surfaces coated with tenascin C exhibited enhanced adhesion and colony formation as mediated by integrin a9b1. In addition, metastatic cells preferentially migrated and colonized tenascin-C–coated trabecular bone xenografts in a novel system that employed chorioallantoic membranes of fertilized chicken eggs as host. Overall, our studies deepen knowledge about reactive stroma responses in the bone endosteum that accompany prostate cancer metastasis to trabecular bone, with potential implications to therapeutically target this process in patients.

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Tenascin-C and integrin α9 mediate interactions of prostate cancer with the bone microenvironment

Abstract

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