Temporal stability of serum concentrations of cytokines and soluble receptors measured across two years in low-risk HIV-seronegative men

Mara M. Epstein, Elizabeth Crabb Breen, Larry Magpantay, Roger Detels, Lauren Lepone, Sudhir Penugonda, Jay Bream, Lisa Paula Jacobson, Otoniel Martínez-Maza, Brenda M. Birmann

Research output: Contribution to journalArticle

Abstract

Background: Prospective cohort studies often quantify serum immune biomarkers at a single time point to determine risk of cancer and other chronic diseases that develop years later. Estimates of the within-person temporal stability of serum markers partly assess the utility of single biomarker measurements and may have important implications for the design of prospective studies of chronic disease risk. Methods: Using archived sera collected from 200 HIV-seronegative men at three visits spaced over approximately 2 years, concentrations of 14 biomarkers (ApoA1, sCD14, sgp130, sIL-6R, sIL-2Rα, sTNFR2, BAFF/BLyS, CXCL13, IFN-γ, interleukin [IL]-1β, IL-6, IL-8, IL-10, and TNF-α) were measured in a single laboratory. Age- And ethnicity-adjusted intraclass correlation coefficients (ICC) were calculated for each biomarker, and mixed linear regression models were used to examine the influence of age, ethnicity, season, and study site on biomarker concentrations. Results: Across all three study visits, most biomarkers had ICC values indicating fair to excellent withinperson stability.ApoA1(ICC=0.88) and TNF-α(ICC=0.87) showed the greatest stability; the ICC for IL-8 (ICC = 0.33) was remarkably less stable. The ICCs were similar when calculated between pairs of consecutive visits. The covariables did not influence biomarker levels or their temporal stability. All biomarkers showed moderate to strong pairwise correlations across visits. Conclusions: Serum concentrations of most evaluatedimmunebiomarkers displayed acceptable to excellent within-person temporal reliability over a 2-year period. Further investigation may be required to clarify the stability of IL-8. Impact: These findings lend support to using these serologic immune biomarkers in prospective studies investigating associations with chronic diseases. investigating associations with chronic diseases.

Original languageEnglish (US)
Pages (from-to)2009-2015
Number of pages7
JournalCancer Epidemiology Biomarkers and Prevention
Volume22
Issue number11
DOIs
StatePublished - Nov 2013

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Cytokine Receptors
Biomarkers
HIV
Serum
Chronic Disease
Interleukin-8
Prospective Studies
Linear Models
Cytokine Receptor gp130
Interleukin-1
Interleukin-10
Immune Sera
Interleukin-6
Cohort Studies

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

Cite this

Temporal stability of serum concentrations of cytokines and soluble receptors measured across two years in low-risk HIV-seronegative men. / Epstein, Mara M.; Breen, Elizabeth Crabb; Magpantay, Larry; Detels, Roger; Lepone, Lauren; Penugonda, Sudhir; Bream, Jay; Jacobson, Lisa Paula; Martínez-Maza, Otoniel; Birmann, Brenda M.

In: Cancer Epidemiology Biomarkers and Prevention, Vol. 22, No. 11, 11.2013, p. 2009-2015.

Research output: Contribution to journalArticle

Epstein, Mara M. ; Breen, Elizabeth Crabb ; Magpantay, Larry ; Detels, Roger ; Lepone, Lauren ; Penugonda, Sudhir ; Bream, Jay ; Jacobson, Lisa Paula ; Martínez-Maza, Otoniel ; Birmann, Brenda M. / Temporal stability of serum concentrations of cytokines and soluble receptors measured across two years in low-risk HIV-seronegative men. In: Cancer Epidemiology Biomarkers and Prevention. 2013 ; Vol. 22, No. 11. pp. 2009-2015.
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T1 - Temporal stability of serum concentrations of cytokines and soluble receptors measured across two years in low-risk HIV-seronegative men

AU - Epstein, Mara M.

AU - Breen, Elizabeth Crabb

AU - Magpantay, Larry

AU - Detels, Roger

AU - Lepone, Lauren

AU - Penugonda, Sudhir

AU - Bream, Jay

AU - Jacobson, Lisa Paula

AU - Martínez-Maza, Otoniel

AU - Birmann, Brenda M.

PY - 2013/11

Y1 - 2013/11

N2 - Background: Prospective cohort studies often quantify serum immune biomarkers at a single time point to determine risk of cancer and other chronic diseases that develop years later. Estimates of the within-person temporal stability of serum markers partly assess the utility of single biomarker measurements and may have important implications for the design of prospective studies of chronic disease risk. Methods: Using archived sera collected from 200 HIV-seronegative men at three visits spaced over approximately 2 years, concentrations of 14 biomarkers (ApoA1, sCD14, sgp130, sIL-6R, sIL-2Rα, sTNFR2, BAFF/BLyS, CXCL13, IFN-γ, interleukin [IL]-1β, IL-6, IL-8, IL-10, and TNF-α) were measured in a single laboratory. Age- And ethnicity-adjusted intraclass correlation coefficients (ICC) were calculated for each biomarker, and mixed linear regression models were used to examine the influence of age, ethnicity, season, and study site on biomarker concentrations. Results: Across all three study visits, most biomarkers had ICC values indicating fair to excellent withinperson stability.ApoA1(ICC=0.88) and TNF-α(ICC=0.87) showed the greatest stability; the ICC for IL-8 (ICC = 0.33) was remarkably less stable. The ICCs were similar when calculated between pairs of consecutive visits. The covariables did not influence biomarker levels or their temporal stability. All biomarkers showed moderate to strong pairwise correlations across visits. Conclusions: Serum concentrations of most evaluatedimmunebiomarkers displayed acceptable to excellent within-person temporal reliability over a 2-year period. Further investigation may be required to clarify the stability of IL-8. Impact: These findings lend support to using these serologic immune biomarkers in prospective studies investigating associations with chronic diseases. investigating associations with chronic diseases.

AB - Background: Prospective cohort studies often quantify serum immune biomarkers at a single time point to determine risk of cancer and other chronic diseases that develop years later. Estimates of the within-person temporal stability of serum markers partly assess the utility of single biomarker measurements and may have important implications for the design of prospective studies of chronic disease risk. Methods: Using archived sera collected from 200 HIV-seronegative men at three visits spaced over approximately 2 years, concentrations of 14 biomarkers (ApoA1, sCD14, sgp130, sIL-6R, sIL-2Rα, sTNFR2, BAFF/BLyS, CXCL13, IFN-γ, interleukin [IL]-1β, IL-6, IL-8, IL-10, and TNF-α) were measured in a single laboratory. Age- And ethnicity-adjusted intraclass correlation coefficients (ICC) were calculated for each biomarker, and mixed linear regression models were used to examine the influence of age, ethnicity, season, and study site on biomarker concentrations. Results: Across all three study visits, most biomarkers had ICC values indicating fair to excellent withinperson stability.ApoA1(ICC=0.88) and TNF-α(ICC=0.87) showed the greatest stability; the ICC for IL-8 (ICC = 0.33) was remarkably less stable. The ICCs were similar when calculated between pairs of consecutive visits. The covariables did not influence biomarker levels or their temporal stability. All biomarkers showed moderate to strong pairwise correlations across visits. Conclusions: Serum concentrations of most evaluatedimmunebiomarkers displayed acceptable to excellent within-person temporal reliability over a 2-year period. Further investigation may be required to clarify the stability of IL-8. Impact: These findings lend support to using these serologic immune biomarkers in prospective studies investigating associations with chronic diseases. investigating associations with chronic diseases.

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