Temporal Shifts in the Dominance of Serotypes of Shigella dysenteriae from 1999 to 2002 in Dhaka, Bangladesh

Kaisar A. Talukder, M. Aminul Islam, Bijay K. Khajanchi, Dilip K. Dutta, Zhahirul Islam, Ashrafus Safa, Khorshed Alam, A. Hossain, G. B. Nair, David A. Sack

Research output: Contribution to journalArticle

Abstract

A total of 358 Shigella dysenteriae strains isolated from patients attending the Dhaka treatment center of the International Centre for Diarrheal Disease Research, Bangladesh, between the years 1999 and 2002 were included in this study. S. dysenteriae type 1, the dominant serotype in 1999 (76.4%), declined to 6.5% in 2002. On the other hand, S. dysenteriae types 2 to 12 were isolated with increasing frequencies of 19, 67, 73.5, and 87% in 1999, 2000, 2001, and 2002, respectively. Of these, types 2 and 4 were the most dominant serotypes, accounting for more than 18.7 and 28.5% of the total isolates, respectively. There was no isolation of serotypes 5, 7, 8, and 13 during this period. Twenty-eight (7.8%) of the isolates were atypical and agglutinated only with the polyvalent antiserum of S. dysenteriae. More than 98% of type 1 strains isolated between 1999 and 2001 were resistant to ampicillin, sulfamethoxazole-trimethoprim, and nalidixic acid. Among other serotypes of S. dysenteriae, Nalr type 2 strains were isolated in 2001 and 2002. Although heterogeneous plasmid profiles were obtained depending on the presence or absence of a single plasmid, core plasmids were defined for particular serotypes. On the other hand, the same plasmid profile was found to be shared by different serotypes. Interestingly, plasmid patterns of types 2 and 4 were almost identical except that a middle-range plasmid of 70 to 60 MDa was present in type 4 in addition to the core plasmids. All the strains harboring the 140-MDa plasmid were positive for the ipaH gene, had Congo red binding abilities, and were positive by the Sereny test, demonstrating their invasive properties.

Original languageEnglish (US)
Pages (from-to)5053-5058
Number of pages6
JournalJournal of Clinical Microbiology
Volume41
Issue number11
DOIs
StatePublished - Nov 2003
Externally publishedYes

Fingerprint

Shigella dysenteriae
Bangladesh
Plasmids
Congo Red
Nalidixic Acid
Serogroup
Sulfamethoxazole Drug Combination Trimethoprim
Ampicillin
Immune Sera

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Temporal Shifts in the Dominance of Serotypes of Shigella dysenteriae from 1999 to 2002 in Dhaka, Bangladesh. / Talukder, Kaisar A.; Islam, M. Aminul; Khajanchi, Bijay K.; Dutta, Dilip K.; Islam, Zhahirul; Safa, Ashrafus; Alam, Khorshed; Hossain, A.; Nair, G. B.; Sack, David A.

In: Journal of Clinical Microbiology, Vol. 41, No. 11, 11.2003, p. 5053-5058.

Research output: Contribution to journalArticle

Talukder, KA, Islam, MA, Khajanchi, BK, Dutta, DK, Islam, Z, Safa, A, Alam, K, Hossain, A, Nair, GB & Sack, DA 2003, 'Temporal Shifts in the Dominance of Serotypes of Shigella dysenteriae from 1999 to 2002 in Dhaka, Bangladesh', Journal of Clinical Microbiology, vol. 41, no. 11, pp. 5053-5058. https://doi.org/10.1128/JCM.41.11.5053-5058.2003
Talukder, Kaisar A. ; Islam, M. Aminul ; Khajanchi, Bijay K. ; Dutta, Dilip K. ; Islam, Zhahirul ; Safa, Ashrafus ; Alam, Khorshed ; Hossain, A. ; Nair, G. B. ; Sack, David A. / Temporal Shifts in the Dominance of Serotypes of Shigella dysenteriae from 1999 to 2002 in Dhaka, Bangladesh. In: Journal of Clinical Microbiology. 2003 ; Vol. 41, No. 11. pp. 5053-5058.
@article{ee479d5c9f3e493b8bf2619d7dd28851,
title = "Temporal Shifts in the Dominance of Serotypes of Shigella dysenteriae from 1999 to 2002 in Dhaka, Bangladesh",
abstract = "A total of 358 Shigella dysenteriae strains isolated from patients attending the Dhaka treatment center of the International Centre for Diarrheal Disease Research, Bangladesh, between the years 1999 and 2002 were included in this study. S. dysenteriae type 1, the dominant serotype in 1999 (76.4{\%}), declined to 6.5{\%} in 2002. On the other hand, S. dysenteriae types 2 to 12 were isolated with increasing frequencies of 19, 67, 73.5, and 87{\%} in 1999, 2000, 2001, and 2002, respectively. Of these, types 2 and 4 were the most dominant serotypes, accounting for more than 18.7 and 28.5{\%} of the total isolates, respectively. There was no isolation of serotypes 5, 7, 8, and 13 during this period. Twenty-eight (7.8{\%}) of the isolates were atypical and agglutinated only with the polyvalent antiserum of S. dysenteriae. More than 98{\%} of type 1 strains isolated between 1999 and 2001 were resistant to ampicillin, sulfamethoxazole-trimethoprim, and nalidixic acid. Among other serotypes of S. dysenteriae, Nalr type 2 strains were isolated in 2001 and 2002. Although heterogeneous plasmid profiles were obtained depending on the presence or absence of a single plasmid, core plasmids were defined for particular serotypes. On the other hand, the same plasmid profile was found to be shared by different serotypes. Interestingly, plasmid patterns of types 2 and 4 were almost identical except that a middle-range plasmid of 70 to 60 MDa was present in type 4 in addition to the core plasmids. All the strains harboring the 140-MDa plasmid were positive for the ipaH gene, had Congo red binding abilities, and were positive by the Sereny test, demonstrating their invasive properties.",
author = "Talukder, {Kaisar A.} and Islam, {M. Aminul} and Khajanchi, {Bijay K.} and Dutta, {Dilip K.} and Zhahirul Islam and Ashrafus Safa and Khorshed Alam and A. Hossain and Nair, {G. B.} and Sack, {David A.}",
year = "2003",
month = "11",
doi = "10.1128/JCM.41.11.5053-5058.2003",
language = "English (US)",
volume = "41",
pages = "5053--5058",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "11",

}

TY - JOUR

T1 - Temporal Shifts in the Dominance of Serotypes of Shigella dysenteriae from 1999 to 2002 in Dhaka, Bangladesh

AU - Talukder, Kaisar A.

AU - Islam, M. Aminul

AU - Khajanchi, Bijay K.

AU - Dutta, Dilip K.

AU - Islam, Zhahirul

AU - Safa, Ashrafus

AU - Alam, Khorshed

AU - Hossain, A.

AU - Nair, G. B.

AU - Sack, David A.

PY - 2003/11

Y1 - 2003/11

N2 - A total of 358 Shigella dysenteriae strains isolated from patients attending the Dhaka treatment center of the International Centre for Diarrheal Disease Research, Bangladesh, between the years 1999 and 2002 were included in this study. S. dysenteriae type 1, the dominant serotype in 1999 (76.4%), declined to 6.5% in 2002. On the other hand, S. dysenteriae types 2 to 12 were isolated with increasing frequencies of 19, 67, 73.5, and 87% in 1999, 2000, 2001, and 2002, respectively. Of these, types 2 and 4 were the most dominant serotypes, accounting for more than 18.7 and 28.5% of the total isolates, respectively. There was no isolation of serotypes 5, 7, 8, and 13 during this period. Twenty-eight (7.8%) of the isolates were atypical and agglutinated only with the polyvalent antiserum of S. dysenteriae. More than 98% of type 1 strains isolated between 1999 and 2001 were resistant to ampicillin, sulfamethoxazole-trimethoprim, and nalidixic acid. Among other serotypes of S. dysenteriae, Nalr type 2 strains were isolated in 2001 and 2002. Although heterogeneous plasmid profiles were obtained depending on the presence or absence of a single plasmid, core plasmids were defined for particular serotypes. On the other hand, the same plasmid profile was found to be shared by different serotypes. Interestingly, plasmid patterns of types 2 and 4 were almost identical except that a middle-range plasmid of 70 to 60 MDa was present in type 4 in addition to the core plasmids. All the strains harboring the 140-MDa plasmid were positive for the ipaH gene, had Congo red binding abilities, and were positive by the Sereny test, demonstrating their invasive properties.

AB - A total of 358 Shigella dysenteriae strains isolated from patients attending the Dhaka treatment center of the International Centre for Diarrheal Disease Research, Bangladesh, between the years 1999 and 2002 were included in this study. S. dysenteriae type 1, the dominant serotype in 1999 (76.4%), declined to 6.5% in 2002. On the other hand, S. dysenteriae types 2 to 12 were isolated with increasing frequencies of 19, 67, 73.5, and 87% in 1999, 2000, 2001, and 2002, respectively. Of these, types 2 and 4 were the most dominant serotypes, accounting for more than 18.7 and 28.5% of the total isolates, respectively. There was no isolation of serotypes 5, 7, 8, and 13 during this period. Twenty-eight (7.8%) of the isolates were atypical and agglutinated only with the polyvalent antiserum of S. dysenteriae. More than 98% of type 1 strains isolated between 1999 and 2001 were resistant to ampicillin, sulfamethoxazole-trimethoprim, and nalidixic acid. Among other serotypes of S. dysenteriae, Nalr type 2 strains were isolated in 2001 and 2002. Although heterogeneous plasmid profiles were obtained depending on the presence or absence of a single plasmid, core plasmids were defined for particular serotypes. On the other hand, the same plasmid profile was found to be shared by different serotypes. Interestingly, plasmid patterns of types 2 and 4 were almost identical except that a middle-range plasmid of 70 to 60 MDa was present in type 4 in addition to the core plasmids. All the strains harboring the 140-MDa plasmid were positive for the ipaH gene, had Congo red binding abilities, and were positive by the Sereny test, demonstrating their invasive properties.

UR - http://www.scopus.com/inward/record.url?scp=10744228143&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10744228143&partnerID=8YFLogxK

U2 - 10.1128/JCM.41.11.5053-5058.2003

DO - 10.1128/JCM.41.11.5053-5058.2003

M3 - Article

C2 - 14605138

AN - SCOPUS:10744228143

VL - 41

SP - 5053

EP - 5058

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 11

ER -