Temporal profile of Src, SSeCKS, and angiogenic factors after focal cerebral ischemia: Correlations with angiogenesis and cerebral edema

Likun Zan, He Wu, Jie Jiang, Shiguang Zhao, Yuejia Song, Guoxin Teng, Heng Li, Ying Jia, Min Zhou, Xin Zhang, Jiping Qi, Jian Wang

Research output: Contribution to journalArticlepeer-review

Abstract

A better understanding of the underlying mechanisms of angiogenesis and vascular permeability is necessary for the development of therapeutic strategies for ischemic injury. The purpose of this study was to examine the spatial and temporal expression of Src and Src-suppressed C kinase substrate (SSeCKS) in brain after middle cerebral artery occlusion (MCAO) and elucidate the relationships among Src, SSeCKS, and the key angiogenic factors present after stroke. Rats were subjected to either MCAO or sham operation. Reverse transcriptase-polymerase chain reaction and Western blotting results revealed that Src gradually increased starting as early as 2 h after MCAO and remained high for 1 day. In contrast, SSeCKS decreased after MCAO. Src expression correlated positively with that of vascular endothelial growth factor and angiopoietin-2, and negatively with that of SSeCKS, angiopoietin-1, and zonula occludens-1. However, SSeCKS had the reverse correlations. Changes in the expression of these factors correlated with the progress of angiogenesis and cerebral edema. Dynamic temporal changes in Src and SSeCKS expression may modulate angiogenesis and cerebral edema formation after focal cerebral ischemia.

Original languageEnglish (US)
Pages (from-to)872-879
Number of pages8
JournalNeurochemistry International
Volume58
Issue number8
DOIs
StatePublished - Jul 2011

Keywords

  • Angiogenesis
  • Cerebral edema
  • Ischemia
  • SSeCKS
  • Src

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

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