Temporal gene expression is restored concomitantly with germ cells in the experimentally regressed rat testis

Jacquetta M. Trasler, Acacia A. Alcivar, Caleb A. Awoniyi, Rosemary Santulli, Barry R Zirkin, Norman B. Hecht

Research output: Contribution to journalArticle

Abstract

The present study was designed to examine the effect of hypophysectomy and subsequent testosterone administration on germ cell numbers and germ cell- and Sertoli cell-specific mRNA levels in adult rats. Rats were hypophysectomized and 4 weeks later received 24-cm testosterone-containing polydimethylsiloxane (PDS) implants. Sham-hypophysectomized rats received an empty PDS implant. At 0 and 3 days, and at 1, 2, 4, and 8 weeks, rats were killed. One testis from each rat (n = 4/group) was used to prepare total RNA; the other testis was used to enumerate stage VII-VIII germ cells. cDNA probes for germ cell and Sertoli cell products were used to monitor germ cell- and Sertoli cell-specific mRNAs on Northern blots. Four weeks after hypophysectomy (0 days), preleptotene and pachytene spermatocytes and round and elongating spermatids were reduced in number to 54%, 12%, 1%, and 0%, respectively, of the control values. Testosterone administration caused a time-dependent increase in germ cell numbers; after 8 weeks of testosterone treatment, preleptotene and pachytene spermatocytes and round and elongating spermatids were 75%, 79%, 74%, and 22%, respectively, of control values. Lactate dehydrogenase-C, phosphoglycerate kinase-2, protamine-1, and sulfated glycoprotein-2 mRNA levels (on a per μg RNA basis) were 34%, 34%, less than 1%, and 580% of control values, respectively, 4 weeks after hypophysectomy and 79%, 87%, 61%, and 192% of control values, respectively, after 8 weeks of testosterone treatment. Pachytene spermatocyte and round spermatid numbers increased, while Sertoli cell sulfated glycoprotein-2 mRNA levels decreased, with respect to 4 week hypophysectomy values, as early as 3 days after implantation of testosterone capsules. In contrast, germ cell (lactate dehydrogenase-C, phosphoglycerate kinase-2, and protamine-1) mRNA levels increased to the greatest extent between 1-4 weeks after the start of testosterone treatment and, after a short lag period, reflected increases in germ cell type and number. The results indicate that cell-specific mRNAs appear concomitantly with germ cell reappearance in a time-dependent manner in the testes of testosterone-treated hypophysectomized adult rats.

Original languageEnglish (US)
Pages (from-to)297-304
Number of pages8
JournalEndocrinology
Volume131
Issue number1
StatePublished - Jul 1992

Fingerprint

Germ Cells
Testis
Testosterone
Gene Expression
Hypophysectomy
Sertoli Cells
Messenger RNA
Spermatocytes
Spermatids
Clusterin
Protamines
Cell Count
RNA
Northern Blotting
Capsules
Complementary DNA

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Trasler, J. M., Alcivar, A. A., Awoniyi, C. A., Santulli, R., Zirkin, B. R., & Hecht, N. B. (1992). Temporal gene expression is restored concomitantly with germ cells in the experimentally regressed rat testis. Endocrinology, 131(1), 297-304.

Temporal gene expression is restored concomitantly with germ cells in the experimentally regressed rat testis. / Trasler, Jacquetta M.; Alcivar, Acacia A.; Awoniyi, Caleb A.; Santulli, Rosemary; Zirkin, Barry R; Hecht, Norman B.

In: Endocrinology, Vol. 131, No. 1, 07.1992, p. 297-304.

Research output: Contribution to journalArticle

Trasler, JM, Alcivar, AA, Awoniyi, CA, Santulli, R, Zirkin, BR & Hecht, NB 1992, 'Temporal gene expression is restored concomitantly with germ cells in the experimentally regressed rat testis', Endocrinology, vol. 131, no. 1, pp. 297-304.
Trasler, Jacquetta M. ; Alcivar, Acacia A. ; Awoniyi, Caleb A. ; Santulli, Rosemary ; Zirkin, Barry R ; Hecht, Norman B. / Temporal gene expression is restored concomitantly with germ cells in the experimentally regressed rat testis. In: Endocrinology. 1992 ; Vol. 131, No. 1. pp. 297-304.
@article{b12a50d18da34985bb25deb715c4954d,
title = "Temporal gene expression is restored concomitantly with germ cells in the experimentally regressed rat testis",
abstract = "The present study was designed to examine the effect of hypophysectomy and subsequent testosterone administration on germ cell numbers and germ cell- and Sertoli cell-specific mRNA levels in adult rats. Rats were hypophysectomized and 4 weeks later received 24-cm testosterone-containing polydimethylsiloxane (PDS) implants. Sham-hypophysectomized rats received an empty PDS implant. At 0 and 3 days, and at 1, 2, 4, and 8 weeks, rats were killed. One testis from each rat (n = 4/group) was used to prepare total RNA; the other testis was used to enumerate stage VII-VIII germ cells. cDNA probes for germ cell and Sertoli cell products were used to monitor germ cell- and Sertoli cell-specific mRNAs on Northern blots. Four weeks after hypophysectomy (0 days), preleptotene and pachytene spermatocytes and round and elongating spermatids were reduced in number to 54{\%}, 12{\%}, 1{\%}, and 0{\%}, respectively, of the control values. Testosterone administration caused a time-dependent increase in germ cell numbers; after 8 weeks of testosterone treatment, preleptotene and pachytene spermatocytes and round and elongating spermatids were 75{\%}, 79{\%}, 74{\%}, and 22{\%}, respectively, of control values. Lactate dehydrogenase-C, phosphoglycerate kinase-2, protamine-1, and sulfated glycoprotein-2 mRNA levels (on a per μg RNA basis) were 34{\%}, 34{\%}, less than 1{\%}, and 580{\%} of control values, respectively, 4 weeks after hypophysectomy and 79{\%}, 87{\%}, 61{\%}, and 192{\%} of control values, respectively, after 8 weeks of testosterone treatment. Pachytene spermatocyte and round spermatid numbers increased, while Sertoli cell sulfated glycoprotein-2 mRNA levels decreased, with respect to 4 week hypophysectomy values, as early as 3 days after implantation of testosterone capsules. In contrast, germ cell (lactate dehydrogenase-C, phosphoglycerate kinase-2, and protamine-1) mRNA levels increased to the greatest extent between 1-4 weeks after the start of testosterone treatment and, after a short lag period, reflected increases in germ cell type and number. The results indicate that cell-specific mRNAs appear concomitantly with germ cell reappearance in a time-dependent manner in the testes of testosterone-treated hypophysectomized adult rats.",
author = "Trasler, {Jacquetta M.} and Alcivar, {Acacia A.} and Awoniyi, {Caleb A.} and Rosemary Santulli and Zirkin, {Barry R} and Hecht, {Norman B.}",
year = "1992",
month = "7",
language = "English (US)",
volume = "131",
pages = "297--304",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "1",

}

TY - JOUR

T1 - Temporal gene expression is restored concomitantly with germ cells in the experimentally regressed rat testis

AU - Trasler, Jacquetta M.

AU - Alcivar, Acacia A.

AU - Awoniyi, Caleb A.

AU - Santulli, Rosemary

AU - Zirkin, Barry R

AU - Hecht, Norman B.

PY - 1992/7

Y1 - 1992/7

N2 - The present study was designed to examine the effect of hypophysectomy and subsequent testosterone administration on germ cell numbers and germ cell- and Sertoli cell-specific mRNA levels in adult rats. Rats were hypophysectomized and 4 weeks later received 24-cm testosterone-containing polydimethylsiloxane (PDS) implants. Sham-hypophysectomized rats received an empty PDS implant. At 0 and 3 days, and at 1, 2, 4, and 8 weeks, rats were killed. One testis from each rat (n = 4/group) was used to prepare total RNA; the other testis was used to enumerate stage VII-VIII germ cells. cDNA probes for germ cell and Sertoli cell products were used to monitor germ cell- and Sertoli cell-specific mRNAs on Northern blots. Four weeks after hypophysectomy (0 days), preleptotene and pachytene spermatocytes and round and elongating spermatids were reduced in number to 54%, 12%, 1%, and 0%, respectively, of the control values. Testosterone administration caused a time-dependent increase in germ cell numbers; after 8 weeks of testosterone treatment, preleptotene and pachytene spermatocytes and round and elongating spermatids were 75%, 79%, 74%, and 22%, respectively, of control values. Lactate dehydrogenase-C, phosphoglycerate kinase-2, protamine-1, and sulfated glycoprotein-2 mRNA levels (on a per μg RNA basis) were 34%, 34%, less than 1%, and 580% of control values, respectively, 4 weeks after hypophysectomy and 79%, 87%, 61%, and 192% of control values, respectively, after 8 weeks of testosterone treatment. Pachytene spermatocyte and round spermatid numbers increased, while Sertoli cell sulfated glycoprotein-2 mRNA levels decreased, with respect to 4 week hypophysectomy values, as early as 3 days after implantation of testosterone capsules. In contrast, germ cell (lactate dehydrogenase-C, phosphoglycerate kinase-2, and protamine-1) mRNA levels increased to the greatest extent between 1-4 weeks after the start of testosterone treatment and, after a short lag period, reflected increases in germ cell type and number. The results indicate that cell-specific mRNAs appear concomitantly with germ cell reappearance in a time-dependent manner in the testes of testosterone-treated hypophysectomized adult rats.

AB - The present study was designed to examine the effect of hypophysectomy and subsequent testosterone administration on germ cell numbers and germ cell- and Sertoli cell-specific mRNA levels in adult rats. Rats were hypophysectomized and 4 weeks later received 24-cm testosterone-containing polydimethylsiloxane (PDS) implants. Sham-hypophysectomized rats received an empty PDS implant. At 0 and 3 days, and at 1, 2, 4, and 8 weeks, rats were killed. One testis from each rat (n = 4/group) was used to prepare total RNA; the other testis was used to enumerate stage VII-VIII germ cells. cDNA probes for germ cell and Sertoli cell products were used to monitor germ cell- and Sertoli cell-specific mRNAs on Northern blots. Four weeks after hypophysectomy (0 days), preleptotene and pachytene spermatocytes and round and elongating spermatids were reduced in number to 54%, 12%, 1%, and 0%, respectively, of the control values. Testosterone administration caused a time-dependent increase in germ cell numbers; after 8 weeks of testosterone treatment, preleptotene and pachytene spermatocytes and round and elongating spermatids were 75%, 79%, 74%, and 22%, respectively, of control values. Lactate dehydrogenase-C, phosphoglycerate kinase-2, protamine-1, and sulfated glycoprotein-2 mRNA levels (on a per μg RNA basis) were 34%, 34%, less than 1%, and 580% of control values, respectively, 4 weeks after hypophysectomy and 79%, 87%, 61%, and 192% of control values, respectively, after 8 weeks of testosterone treatment. Pachytene spermatocyte and round spermatid numbers increased, while Sertoli cell sulfated glycoprotein-2 mRNA levels decreased, with respect to 4 week hypophysectomy values, as early as 3 days after implantation of testosterone capsules. In contrast, germ cell (lactate dehydrogenase-C, phosphoglycerate kinase-2, and protamine-1) mRNA levels increased to the greatest extent between 1-4 weeks after the start of testosterone treatment and, after a short lag period, reflected increases in germ cell type and number. The results indicate that cell-specific mRNAs appear concomitantly with germ cell reappearance in a time-dependent manner in the testes of testosterone-treated hypophysectomized adult rats.

UR - http://www.scopus.com/inward/record.url?scp=0026693211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026693211&partnerID=8YFLogxK

M3 - Article

C2 - 1612009

AN - SCOPUS:0026693211

VL - 131

SP - 297

EP - 304

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 1

ER -