Temporal events in skin injury and the early adaptive responses in ultraviolet-irradiated mouse skin

Allal Ouhtit, H. Konrad Muller, Darren W. Davis, Stephen E. Ullrich, David McConkey, Honnavara N. Ananthaswamy

Research output: Contribution to journalArticle

Abstract

We examined the effects of ultraviolet (UV) radiation on the time course for induction of sunburn (apoptotic) cells and expression of proteins known to be associated with growth arrest and apoptosis in SKH-hr1 mouse skin. Mice were irradiated with a single dose (2.5 kJ/m2) of UV from Kodacel-filtered (290-400 nm) FS40 sunlamps and the skin tissues were analyzed at various times after irradiation for the presence of apoptotic cells and expression of p53, p21(Waf-1/cip1), bcl-2, bax, and proliferating cell nuclear antigen. The results indicated that p53 expression was induced early in the epidermis, reaching maximum levels 12 hours after irradiaton, and p21(Waf-1/Cip1) expression in the epidermis peaked at 24 hours after irradiation. In contrast, UV radiation induced high levels of bax at 24 to 72 hours after irradiation with a concomitant decrease in bcl-2 expression. Coinciding with these changes, apoptotic cells began to appear 6 hours after irradiation and reached a maximum at 24 hours after irradiation. Interestingly, proliferating cell nuclear antigen expression, which was initially confined to the basal layer, became dispersed throughout the basal and suprabasal layers of the skin at 48 hours and paralleled marked hyperplasia. These results suggest that UV irradiation of mouse skin induces apoptosis mediated by the p53/p21/bax/bcl-2 pathway and that the dead cells are replaced by hyperproliferative cells, leading to epidermal hyperplasia. This implies that UV-induced apoptosis and hyperplasia are closely linked and tightly regulated and that dysregulation of these two events may lead to skin cancer development.

Original languageEnglish (US)
Pages (from-to)201-207
Number of pages7
JournalAmerican Journal of Pathology
Volume156
Issue number1
DOIs
StatePublished - 2000
Externally publishedYes

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Skin
Wounds and Injuries
Hyperplasia
Proliferating Cell Nuclear Antigen
Apoptosis
Epidermis
Sunburn
Radiation Effects
Skin Neoplasms
Radiation
Growth
Proteins

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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Temporal events in skin injury and the early adaptive responses in ultraviolet-irradiated mouse skin. / Ouhtit, Allal; Muller, H. Konrad; Davis, Darren W.; Ullrich, Stephen E.; McConkey, David; Ananthaswamy, Honnavara N.

In: American Journal of Pathology, Vol. 156, No. 1, 2000, p. 201-207.

Research output: Contribution to journalArticle

Ouhtit, Allal ; Muller, H. Konrad ; Davis, Darren W. ; Ullrich, Stephen E. ; McConkey, David ; Ananthaswamy, Honnavara N. / Temporal events in skin injury and the early adaptive responses in ultraviolet-irradiated mouse skin. In: American Journal of Pathology. 2000 ; Vol. 156, No. 1. pp. 201-207.
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AB - We examined the effects of ultraviolet (UV) radiation on the time course for induction of sunburn (apoptotic) cells and expression of proteins known to be associated with growth arrest and apoptosis in SKH-hr1 mouse skin. Mice were irradiated with a single dose (2.5 kJ/m2) of UV from Kodacel-filtered (290-400 nm) FS40 sunlamps and the skin tissues were analyzed at various times after irradiation for the presence of apoptotic cells and expression of p53, p21(Waf-1/cip1), bcl-2, bax, and proliferating cell nuclear antigen. The results indicated that p53 expression was induced early in the epidermis, reaching maximum levels 12 hours after irradiaton, and p21(Waf-1/Cip1) expression in the epidermis peaked at 24 hours after irradiation. In contrast, UV radiation induced high levels of bax at 24 to 72 hours after irradiation with a concomitant decrease in bcl-2 expression. Coinciding with these changes, apoptotic cells began to appear 6 hours after irradiation and reached a maximum at 24 hours after irradiation. Interestingly, proliferating cell nuclear antigen expression, which was initially confined to the basal layer, became dispersed throughout the basal and suprabasal layers of the skin at 48 hours and paralleled marked hyperplasia. These results suggest that UV irradiation of mouse skin induces apoptosis mediated by the p53/p21/bax/bcl-2 pathway and that the dead cells are replaced by hyperproliferative cells, leading to epidermal hyperplasia. This implies that UV-induced apoptosis and hyperplasia are closely linked and tightly regulated and that dysregulation of these two events may lead to skin cancer development.

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