Diferencias temporales en la detección de fuentes de alimentación en el contenido intestinal de Triatoma infestans

Translated title of the contribution: Temporal differences in blood meal detection from the midguts of Triatoma infestans

Jesus Pinto, Dawn M. Roellig, Robert H Gilman, Maritza Calderón, Carlos Bartra, Renzo Salazar, Caryn Bern, Jenny Ancca-Juárez, Michael Levy, Cesar Náquira, Vitaliano Cama

Research output: Contribution to journalArticle

Abstract

We used genus/species specific PCRs to determine the temporal persistence of host DNA in Triatoma infestans experimentally fed on blood from six common vertebrate species: humans, domestic dogs, guinea pigs, chickens, mice, and pigs. Twenty third or fourth instar nymphs per animal group were allowed to feed to engorgement, followed by fasting-maintenance in the insectary. At 7, 14, 21, or 28 days post-feeding, the midgut contents from five triatomines per group were tested with the respective PCR assay. DNA from all vertebrate species was detected in at least four of five study nymphs at seven and 14 days post-feeding. DNA of humans, domestic dogs, guinea pigs, pigs, and chickens were more successfully detected (80-100%) through day 21, and less successfully (20-100%) at day 28. Findings demonstrate that species-specific PCRs can consistently identify feeding sources of T. infestans within two weeks, a biologically relevant time interval.

Original languageSpanish
Pages (from-to)83-87
Number of pages5
JournalRevista do Instituto de Medicina Tropical de Sao Paulo
Volume54
Issue number2
DOIs
Publication statusPublished - Mar 2012

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Keywords

  • Blood meal analysis
  • Chagas disease
  • PCR
  • Triatoma infestans
  • Trypanosoma cruzi

ASJC Scopus subject areas

  • Infectious Diseases

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