Temporal changes in neurotrophic factors and neurite outgrowth in the major pelvic ganglion following cavernous nerve injury

Johanna L. Hannan, Maarten Albersen, Bernard L. Stopak, Xiaopu Liu, Arthur Burnett, Ahmet Hoke, Trinity Bivalacqua

Research output: Contribution to journalArticle

Abstract

Despite nerve-sparing radical prostatectomy, nerve damage and erectile dysfunction (ED) prevail, and preventing neurodegeneration is of great importance. Neurotrophic factors and neurite outgrowth were characterized in major pelvic ganglia (MPG) following bilateral cavernous nerve injury (BCNI). Young male Sprague-Dawley rats underwent sham or BCNI surgery, and the intracavernosal pressure to mean arterial pressure ratio was measured 2, 7, 14, 21, 30, and 60 days following injury (n=8/group). MPG gene expression (qPCR) and Western blot were performed for glial cell line-derived neurotrophic factor (GDNF), nerve growth factor (NGF), neurturin, neurotrophin (NT)-3, NT4, brain-derived neurotrophic factor (BDNF), vascular endothelial growth factor, and activating transcription factor 3 (ATF3). Additional rats were injured, and MPGs were removed 24 hr, 48 hr, 3 days, and 7 days following BCNI (n=3/group). MPGs were cultured in Matrigel, and neurite outgrowth was measured. Erections were impaired early and improved by 60 days in BCNI rats. GDNF, NGF, BDNF, and ATF3 gene expression was significantly increased and NT3 was decreased in MPGs following BCNI (48 hr to 21 days, P

Original languageEnglish (US)
Pages (from-to)954-963
Number of pages10
JournalJournal of Neuroscience Research
Volume93
Issue number6
DOIs
StatePublished - Jun 1 2015

Fingerprint

Nerve Growth Factors
Ganglia
Wounds and Injuries
Activating Transcription Factor 3
Glial Cell Line-Derived Neurotrophic Factor
Brain-Derived Neurotrophic Factor
Nerve Growth Factor
Neurturin
Neurotrophin 3
Gene Expression
Erectile Dysfunction
Prostatectomy
Vascular Endothelial Growth Factor A
Sprague Dawley Rats
Neuronal Outgrowth
Arterial Pressure
Western Blotting
Pressure

Keywords

  • Activating transcription factor 3
  • Erectile dysfunction
  • Neurite outgrowth
  • Peripheral nerve injury

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Temporal changes in neurotrophic factors and neurite outgrowth in the major pelvic ganglion following cavernous nerve injury. / Hannan, Johanna L.; Albersen, Maarten; Stopak, Bernard L.; Liu, Xiaopu; Burnett, Arthur; Hoke, Ahmet; Bivalacqua, Trinity.

In: Journal of Neuroscience Research, Vol. 93, No. 6, 01.06.2015, p. 954-963.

Research output: Contribution to journalArticle

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