Temporal Acquisition of Enhanced Fibrinolytic Activity by Syrian Hamster Embryo Cells following Treatment with Benzo(a)pyrene

Peter A. Jones, William F. Benedict, Deborah L. Grady, Lynda D. Hester

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55 Scopus citations

Abstract

Following treatment of Syrian hamster embryo cells with benzo(a)pyrene, the time required for the expression of enhanced fibrinolytic activity was examined. For this study, the fibrin-agarose overlay method was developed to distinguish the activity of normal and transformed colonies of hamster cells. Colonies possessing enhanced fibrinolytic activity were not observed 8 days after treatment, but they were observed following one passage (2 weeks after treatment). Morphologically transformed colonies, which exhibited no enhanced fibrinolytic activity, were observed 8 days following treatment. In contrast to these two early changes, cells capable of growth in soft agar were observed much later (6 to 8 weeks after treatment). Untreated Syrian hamster embryo cells generally senesced and did not exhibit enhanced fibrinolytic activity. Approximately 1 of 10 untreated cultures escaped senescence and evolved as a continuous cell line; such cultures frequently exhibited enhanced fibrinolytic activity. These results suggest that the acquisition of enhanced fibrinolytic activity, while perhaps not a cause of neoplastic transformation, may reflect a loss of control of the normal function of the cellular genetic apparatus during the process of transformation.

Original languageEnglish (US)
Pages (from-to)3815-3823
Number of pages9
JournalCancer Research
Volume37
Issue number10
StatePublished - Oct 1977

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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