Temperature is a powerful promoter of interleukin 2 transcription

James E. Gern, John R. Jayman, Lisa I. Goldberg, Patrick A. Murphy, Howard M Lederman

Research output: Contribution to journalArticle

Abstract

Elevated temperature has profound effects on the immune system, particularly by increasing T-cell proliferation rates, interleukin 1 (IL-1)-driven secretion of IL-2, and primary antibody responses to T-dependent antigens. Therefore, this study shows, in detail, the effects of incubation temperature (29°C to 41°C) on proliferation, IL-2 secretion, and IL-2 mRNA expression in both a murine thymoma cell line (EL4-6.1) and in nontransformed murine splenocytes. Temperature was found to be a positive regulator of IL-2 secretion whether or not IL-1 was part of the activation signal. Parallel effects were observed at the level of IL-2 gene expression. Messenger RNA was quantitated with a novel system, using solution hybridization followed by detection of RNA-DNA complexes by enzyme immunoassay. The time to onset of IL-2 mRNA expression was inversely related to temperature, and mRNA levels increased 20- to 50-fold with increases in average incubation temperature from 29°C to 39°C. This effect was observed whether cells were incubated at constant temperature or exposed intermittently to elevated temperature. Over the same intervals of time and temperature, mRNA levels for τ-actin and β-tubulin remained relatively constant. Taken together, these findings suggest that temperature-mediated augmentation of IL-2 secretion does not require the presence of IL-1, and that the effect occurs at a pretranslational level.

Original languageEnglish (US)
Pages (from-to)389-397
Number of pages9
JournalCytokine
Volume3
Issue number5
DOIs
StatePublished - 1991

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Transcription
Interleukin-2
Temperature
Messenger RNA
Interleukin-1
Thymoma
T-cells
Immune system
Viral Tumor Antigens
Cell proliferation
Tubulin
Immunoenzyme Techniques
Gene expression
Antibody Formation
Actins
Immune System
Chemical activation
Cells
Cell Proliferation
RNA

Keywords

  • IL-2
  • temperature
  • transcription

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

Temperature is a powerful promoter of interleukin 2 transcription. / Gern, James E.; Jayman, John R.; Goldberg, Lisa I.; Murphy, Patrick A.; Lederman, Howard M.

In: Cytokine, Vol. 3, No. 5, 1991, p. 389-397.

Research output: Contribution to journalArticle

Gern, James E. ; Jayman, John R. ; Goldberg, Lisa I. ; Murphy, Patrick A. ; Lederman, Howard M. / Temperature is a powerful promoter of interleukin 2 transcription. In: Cytokine. 1991 ; Vol. 3, No. 5. pp. 389-397.
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