TY - JOUR
T1 - Temperature dependence of interaction between double stranded DNA and Cy3 or Cy5
AU - Li, Xun
AU - Yin, Yandong
AU - Yang, Xinxing
AU - Zhi, Zeyong
AU - Zhao, Xin Sheng
N1 - Funding Information:
We thank Prof. David Lilley for helpful discussion. This work was supported by NSFC ( 20733001, 20973015 ) and by NKBRSF ( 2006CB910300, 2010CB912302 ).
PY - 2011/9/15
Y1 - 2011/9/15
N2 - Fluorescence resonance energy transfer (FRET) has been widely used as molecular rulers to measure the distance in biomolecules. In this Letter, double stranded DNA (dsDNA) with Cy3 and Cy5 labeled on the 5′-terminals of each strand is used to investigate how temperature influences the interaction between dsDNA and Cy3/Cy5. By applying the orientation-dependent model to fit our FRET data, we found that the interaction between dsDNA and Cy3/Cy5 is so strong that Cy3 and Cy5 are mostly capped on the end of dsDNA duplex in temperature range of 4-50 °C. So, the orientation factor has to be cautiously considered when using FRET to measure distance. Meanwhile, with the increase of temperature, the orientational randomization of the dyes becomes more prominent.
AB - Fluorescence resonance energy transfer (FRET) has been widely used as molecular rulers to measure the distance in biomolecules. In this Letter, double stranded DNA (dsDNA) with Cy3 and Cy5 labeled on the 5′-terminals of each strand is used to investigate how temperature influences the interaction between dsDNA and Cy3/Cy5. By applying the orientation-dependent model to fit our FRET data, we found that the interaction between dsDNA and Cy3/Cy5 is so strong that Cy3 and Cy5 are mostly capped on the end of dsDNA duplex in temperature range of 4-50 °C. So, the orientation factor has to be cautiously considered when using FRET to measure distance. Meanwhile, with the increase of temperature, the orientational randomization of the dyes becomes more prominent.
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U2 - 10.1016/j.cplett.2011.08.017
DO - 10.1016/j.cplett.2011.08.017
M3 - Article
AN - SCOPUS:80052448292
SN - 0009-2614
VL - 513
SP - 271
EP - 275
JO - Chemical Physics Letters
JF - Chemical Physics Letters
IS - 4-6
ER -