Telomere stability genes are not mutated in osteosarcoma cell lines

Sharon A. Savage, Brian J. Stewart, Jason S. Liao, Lee J. Helman, Stephen J. Chanock

Research output: Contribution to journalArticlepeer-review


Osteosarcoma (OS), the most common primary bone tumor in adolescents and young adults, is characterized by a high degree of chromosomal abnormalities. Because telomeres are important for maintaining chromosomal integrity, it is plausible that germ-line or somatic mutations in the genes responsible for stabilizing the telomere complex could contribute to OS. We performed bi-directional sequence analysis in five OS cell lines and targeted all exons and proximal promoter regions in eight genes important in telomere stability: telomerase, the RNA component of telomerase (TERC), telomeric repeat binding factor 1, telomeric repeat binding factor 2, TERF1 interacting nuclear factor 2, human Rap1, protection of telomeres 1 and tankyrase. In this pilot study, we did not identify either somatic mutations or novel germ-line mutations in the five cell lines studied. However, we did confirm common genetic polymorphisms; an analysis of heterozygous sites suggests that loss of heterozygosity in OS is not present across these eight genes.

Original languageEnglish (US)
Pages (from-to)79-81
Number of pages3
JournalCancer Genetics and Cytogenetics
Issue number1
StatePublished - Jul 1 2005

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research


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