Telomere shortening and chromosomal instability abrogates proliferation of adult but not embryonic neural stem cells

Sacri Ferrón, Helena Mira, Sonia Franco, Marifé Cano-Jimenez, Elena Bellmunt, Carmen Ramírez, Isabel Fariñas, María A. Blasco

Research output: Contribution to journalArticle

Abstract

Chromosome integrity is essential for cell viability and, therefore, highly proliferative cell types require active telomere elongation mechanisms to grow indefinitely. Consistently, deletion of telomerase activity in a genetically modified mouse strain results in growth impairments in all highly proliferative cell populations analyzed so far. We show that telomere attrition dramatically impairs the in vitro proliferation of adult neural stem cells (NSCs) isolated from the subventricular zone (SVZ) of telomerase-deficient adult mice. Reduced proliferation of postnatal neurogenic progenitors was also observed in vivo, in the absence of exogenous mitogenic stimulation. Strikingly, severe telomere erosion resulting in chromosomal abnormalities and nuclear accumulation of p53 did not affect the in vitro proliferative potential of embryonic NSCs. These results suggest that intrinsic differences exist between embryonic and adult neural progenitor cells in their response to telomere shortening, and that some populations of tissue-specific stem cells can bypass DNA damage check points.

Original languageEnglish (US)
Pages (from-to)4059-4070
Number of pages12
JournalDevelopment
Volume131
Issue number16
DOIs
StatePublished - Aug 2004
Externally publishedYes

Fingerprint

Telomere Shortening
Chromosomal Instability
Neural Stem Cells
Telomere
Embryonic Stem Cells
Telomerase
Stem Cells
Adult Stem Cells
Lateral Ventricles
Chromosome Aberrations
Population
DNA Damage
Cell Survival
Chromosomes
Growth
In Vitro Techniques

Keywords

  • Differentiation
  • Neural progenitor
  • Neurogenesis
  • Telomerase knockout

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Ferrón, S., Mira, H., Franco, S., Cano-Jimenez, M., Bellmunt, E., Ramírez, C., ... Blasco, M. A. (2004). Telomere shortening and chromosomal instability abrogates proliferation of adult but not embryonic neural stem cells. Development, 131(16), 4059-4070. https://doi.org/10.1242/dev.01215

Telomere shortening and chromosomal instability abrogates proliferation of adult but not embryonic neural stem cells. / Ferrón, Sacri; Mira, Helena; Franco, Sonia; Cano-Jimenez, Marifé; Bellmunt, Elena; Ramírez, Carmen; Fariñas, Isabel; Blasco, María A.

In: Development, Vol. 131, No. 16, 08.2004, p. 4059-4070.

Research output: Contribution to journalArticle

Ferrón, S, Mira, H, Franco, S, Cano-Jimenez, M, Bellmunt, E, Ramírez, C, Fariñas, I & Blasco, MA 2004, 'Telomere shortening and chromosomal instability abrogates proliferation of adult but not embryonic neural stem cells', Development, vol. 131, no. 16, pp. 4059-4070. https://doi.org/10.1242/dev.01215
Ferrón S, Mira H, Franco S, Cano-Jimenez M, Bellmunt E, Ramírez C et al. Telomere shortening and chromosomal instability abrogates proliferation of adult but not embryonic neural stem cells. Development. 2004 Aug;131(16):4059-4070. https://doi.org/10.1242/dev.01215
Ferrón, Sacri ; Mira, Helena ; Franco, Sonia ; Cano-Jimenez, Marifé ; Bellmunt, Elena ; Ramírez, Carmen ; Fariñas, Isabel ; Blasco, María A. / Telomere shortening and chromosomal instability abrogates proliferation of adult but not embryonic neural stem cells. In: Development. 2004 ; Vol. 131, No. 16. pp. 4059-4070.
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