Telomere position effect in human cells

J. A. Baur, Y. Zou, J. W. Shay, W. E. Wright

Research output: Contribution to journalArticlepeer-review

Abstract

In yeast, telomere position effect (TPE) results in the reversible silencing of genes near telomeres. Here we demonstrate the presence of TPE in human cells. HeLa clones containing a luciferase reporter adjacent to a newly formed telomere express 10 times less luciferase than do control clones generated by random integration. Luciferase expression is restored by trichostatin A, a histone deacetylase inhibitor. Overexpression of a human telomerase reverse transcriptase complementary DNA results in telomere elongation and an additional 2- to 10-fold decrease in expression in telomeric dones but not control clones. The dependence of TPE on telomere length provides a mechanism for the modification of gene expression throughout the replicative life-span of human cells.

Original languageEnglish (US)
Pages (from-to)2075-2077
Number of pages3
JournalScience
Volume292
Issue number5524
DOIs
StatePublished - Jun 15 2001
Externally publishedYes

ASJC Scopus subject areas

  • General

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