Telomere length control and transcriptional regulation of subtelomeric adhesins in Candida glabrata

Irene Castaño, Shih Jung Pan, Margaret Zupancic, Christophe Hennequin, Bernard Dujon, Brendan P. Cormack

Research output: Contribution to journalArticlepeer-review

Abstract

The pathogenic yeast Candida glabrata is able to bind in vitro to human epithelial cells. This interaction depends on expression of the adhesin Epa1p. The genome contains a number of EPA1 paralogues which localize to the subtelomeric regions of the C. glabrata. We have identified three hyperadherent mutants of C. glabrata. The first has an insertion adjacent to EPA7, an EPA1-related adhesin. The others disrupt the SIR3 and RIF1 genes of C. glabrata. We show that SIR3 and RIF1 are required for subtelomeric silencing in C. glabrata and that RIF1 regulates telomere length in C. glabrata. We show that the hyperadherent phenotype of the sir3Δ and rif1Δ deletion strains depends primarily on derepression of two novel members of the EPA gene family - EPA6 and EPA7. The sir-3Δ and rif1Δ mutants show increased colonization of the kidney in a murine model of disseminated infection and this hypercolonization depends, at least in part, on derepression of EPA6 and EPA7. The analysis here is the first evidence that multiple EPA genes encode adhesins and demonstrates that transcription of at least two of these adhesins is regulated by subtelomeric silencing.

Original languageEnglish (US)
Pages (from-to)1246-1258
Number of pages13
JournalMolecular Microbiology
Volume55
Issue number4
DOIs
StatePublished - Feb 2005

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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