We showed that telomerase activity (TAC) of human promyelocytic leukemic cells U-937 and HL-60 sharply decreased after induction of macrophagal differentiation. Few days after inductor removal cledifferentiation occured that was accompanied by proliferation resumption and TAC increase. TAC significantly decreased also in the case of U-937 cell proliferation cessation in the course of long-term azidothymidine inhibition of telomerase function. A slow decrease in TAC during 6-8 days was observed in the course of transition of murine fibroblasts 3T3 Swiss to the quiescent state. TAC decreased in serum-deprived cells, as well as in slowly proliferated high-density inhibited cells. During the quiescence exit and dedifferentiation, the increase in TAC preceded proliferation resumption. In all the described cases we observed correlations between TAC alterations and alterations in the nonspecific polymerase activity that we had found out earlier (Chernov D.N., Yegorov Y.E., Akimov S.S.// Doklady Biochemistry. 1996. V. 349. P. 55-58). The problems of TAC regulation are discussed.
|Original language||English (US)|
|Number of pages||2|
|State||Published - Dec 1 1998|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology