Telomerase activity in germline and embryonic cells of Xenopus

L. L. Mantell, C. W. Greider

Research output: Contribution to journalArticle

Abstract

Telomerase is a ribonucleoprotein which synthesizes telomere repeats onto chromosome ends. Telomerase activity is involved in telomere length maintenance. We used Xenopus laevis as a model system to study the expression of telomerase activity in germline cells and during early development. We identified a nonprocessive telomerase activity in manually dissected nuclei of Xenopus stage VI oocytes. Telomerase activity was detected throughout oogenesis and embryogenesis. Telomerase was active in both S and M phase cell cycle extracts, suggesting that telomerase activity is not regulated with chromosomal DNA replication.

Original languageEnglish (US)
Pages (from-to)3211-3217
Number of pages7
JournalEMBO Journal
Volume13
Issue number13
DOIs
StatePublished - 1994

Keywords

  • Cell cycle
  • Embryogenesis
  • Germline
  • Oogenesis
  • Telomerase

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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