Telomerase activity in germ cell cancers and mature teratomas

Juan Albanell, George J. Bosl, Victor E. Reuter, Monika Engelhardt, Sonia Franco, Malcolm A S Moore, Ethan Dmitrovsky

Research output: Contribution to journalArticle

Abstract

Background: An inverse relationship has been reported between the presence of telomerase enzymatic activity and the induction of differentiation in human tumor cell lines. Male germ cell tumors represent an attractive clinical model to assess this relationship further because high telomerase activity is present in normal germ cell progenitors and in embryonal carcinomas that can differentiate into mature teratomas. To investigate how telomerase activity and the differentiation state of germ cell tumors are related, telomerase activities and telomere lengths were measured in benign testicular tissues, germ cell cancers, and mature or immature teratomas. Methods: By use of a modified telomeric repeat amplification protocol (TRAP) assay, telomerase activity was measured in four specimens of benign testicular tissue, in 27 germ cell cancers, in seven mature teratomas, and in one immature teratoma. Telomere lengths were measured in all specimens by restriction digestion of genomic DNA and Southern blot hybridization analysis. Associations between telomerase activity and tissue histopathology were assessed with two-sided Fisher's exact tests. Results: Telomerase activity was detected in all examined germ cell cancers and in the benign testicular tissue specimens. In marked contrast, telomerase activity was not detected in any mature teratoma (P

Original languageEnglish (US)
Pages (from-to)1321-1326
Number of pages6
JournalJournal of the National Cancer Institute
Volume91
Issue number15
StatePublished - Aug 4 1999
Externally publishedYes

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Germ Cell and Embryonal Neoplasms
Telomerase
Teratoma
Telomere
Embryonal Carcinoma
Southern Blotting
Tumor Cell Line
Germ Cells
Digestion
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Albanell, J., Bosl, G. J., Reuter, V. E., Engelhardt, M., Franco, S., Moore, M. A. S., & Dmitrovsky, E. (1999). Telomerase activity in germ cell cancers and mature teratomas. Journal of the National Cancer Institute, 91(15), 1321-1326.

Telomerase activity in germ cell cancers and mature teratomas. / Albanell, Juan; Bosl, George J.; Reuter, Victor E.; Engelhardt, Monika; Franco, Sonia; Moore, Malcolm A S; Dmitrovsky, Ethan.

In: Journal of the National Cancer Institute, Vol. 91, No. 15, 04.08.1999, p. 1321-1326.

Research output: Contribution to journalArticle

Albanell, J, Bosl, GJ, Reuter, VE, Engelhardt, M, Franco, S, Moore, MAS & Dmitrovsky, E 1999, 'Telomerase activity in germ cell cancers and mature teratomas', Journal of the National Cancer Institute, vol. 91, no. 15, pp. 1321-1326.
Albanell J, Bosl GJ, Reuter VE, Engelhardt M, Franco S, Moore MAS et al. Telomerase activity in germ cell cancers and mature teratomas. Journal of the National Cancer Institute. 1999 Aug 4;91(15):1321-1326.
Albanell, Juan ; Bosl, George J. ; Reuter, Victor E. ; Engelhardt, Monika ; Franco, Sonia ; Moore, Malcolm A S ; Dmitrovsky, Ethan. / Telomerase activity in germ cell cancers and mature teratomas. In: Journal of the National Cancer Institute. 1999 ; Vol. 91, No. 15. pp. 1321-1326.
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AU - Reuter, Victor E.

AU - Engelhardt, Monika

AU - Franco, Sonia

AU - Moore, Malcolm A S

AU - Dmitrovsky, Ethan

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