Telomerase activity as a measure for monitoring radiocurability of tumor cells

Satin G. Sawant, Vincent Gregoire, Sonu Dhar, Christopher B. Umbricht, Sophia Cvilic, Saraswati Sukumar, Tej K. Pandita

Research output: Contribution to journalArticle

Abstract

Radiotherapy plays a key role in the treatment of many tumors. It is difficult to determine what fraction of tumor cells survives after treatment with ionizing radiation. A convenient and sensitive biochemical assay could be efficacious in determining the potential success of radiotherapy. Since telomerase activity is frequently associated with the malignant phenotype, we sought to determine whether a correlation existed between ionizing radiation- induced cell killing and telomerase activity. We evaluated telomerase activity in two telomerase-positive and one telomerase-negative human cell line exposed to ionizing radiation. Telomerase activity was determined using a PCR-based telomeric repeat amplification protocol coupled with ELISA. We found ionizing radiation treatment to decrease the telomerase activity (in plateau phase cells of RKO, HeLa; and growing cells of RKO) in a dose- dependent manner, which correlated with cell death in in vitro tests as well as during tumor regression in nude mice. In contrast, growing HeLa cells after 24 h postradiation treatment showed an increase in telomerase activity, but there was no increase in the levels of mRNA of hTERT. To assess the sensitivity of the telomerase activity assay, we performed mixing experiments of HeLa and AG1522 cell extracts. These studies showed that telomerase activity could be detected in lysate equal to a single HeLa cell when mixed with 10,000 AG1522 cells. Our results indicate that even a few surviving neoplastic cells can be detected by telomerase activity assay. Therefore, detection of telomerase activity may be a useful monitor of radiotherapeutic efficacy and an early predictor of outcome.

Original languageEnglish (US)
Pages (from-to)1047-1054
Number of pages8
JournalFASEB Journal
Volume13
Issue number9
StatePublished - Jun 8 1999

Keywords

  • Cell kill
  • Ionizing radiation
  • Tumor growth

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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  • Cite this

    Sawant, S. G., Gregoire, V., Dhar, S., Umbricht, C. B., Cvilic, S., Sukumar, S., & Pandita, T. K. (1999). Telomerase activity as a measure for monitoring radiocurability of tumor cells. FASEB Journal, 13(9), 1047-1054.