Technique for false image correction in second harmonic generation microscopy by modulating laser polarization

L. Jin; L. Gao; L. Huo; W. Yuan; Y. H. Luo; A. H.P. Hot; C. Lint

doi:10.1109/METAMAT.2006.335001

Technique for false image correction in second harmonic generation microscopy by modulating laser polarization

L. Jin, L. Gao, L. Huo, W. Yuan, Y. H. Luo, A. H.P. Hot, C. Lint

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Scopus citations

Abstract

Second harmonic generation microscopy (SHGM) has been widely used in biomedicine as a promising non-invasive imaging technique. However, due to the complexity of biological samples, false images are often produced depending on sample orientation relative to laser polarization and on sample parameter related to the second-order optical nonlinear susceptibility. In this work, we present a convenient SHG technique to correct the false images by modulating laser polarization and stitching complementary images together, the stitched image will contain more detailed information and will better represent the sample structures. We first describe our SHG technique with a brief mathematical model, and then show its validity by performing it on two kind of samples: symmetrically synthesized bio-origin polymer poly(3-hyroxybutyrate-co-3- hydroxyhexanoate) (PHBHHx) and fibrillar collagen of rat-tail tendon.

Original language	English (US)
Title of host publication	Proceedings of International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006
Publisher	IEEE Computer Society
Pages	72-75
Number of pages	4
ISBN (Print)	0780397746, 9780780397743
DOIs	https://doi.org/10.1109/METAMAT.2006.335001
State	Published - 2006
Externally published	Yes
Event	International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006 - Hangzhou, China Duration: Oct 16 2006 → Oct 18 2006

Publication series

Name	Proceedings of International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006

Other

Other	International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006
Country/Territory	China
City	Hangzhou
Period	10/16/06 → 10/18/06

ASJC Scopus subject areas

Biotechnology
Biomaterials
Electronic, Optical and Magnetic Materials

Access to Document

10.1109/METAMAT.2006.335001

Cite this

Jin, L., Gao, L., Huo, L., Yuan, W., Luo, Y. H., Hot, A. H. P., & Lint, C. (2006). Technique for false image correction in second harmonic generation microscopy by modulating laser polarization. In Proceedings of International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006 (pp. 72-75). Article 4134740 (Proceedings of International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006). IEEE Computer Society. https://doi.org/10.1109/METAMAT.2006.335001

Technique for false image correction in second harmonic generation microscopy by modulating laser polarization. / Jin, L.; Gao, L.; Huo, L. et al.
Proceedings of International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006. IEEE Computer Society, 2006. p. 72-75 4134740 (Proceedings of International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Jin, L, Gao, L, Huo, L, Yuan, W, Luo, YH, Hot, AHP & Lint, C 2006, Technique for false image correction in second harmonic generation microscopy by modulating laser polarization. in Proceedings of International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006., 4134740, Proceedings of International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006, IEEE Computer Society, pp. 72-75, International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006, Hangzhou, China, 10/16/06. https://doi.org/10.1109/METAMAT.2006.335001

Jin L, Gao L, Huo L, Yuan W, Luo YH, Hot AHP et al. Technique for false image correction in second harmonic generation microscopy by modulating laser polarization. In Proceedings of International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006. IEEE Computer Society. 2006. p. 72-75. 4134740. (Proceedings of International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006). doi: 10.1109/METAMAT.2006.335001

Jin, L. ; Gao, L. ; Huo, L. et al. / Technique for false image correction in second harmonic generation microscopy by modulating laser polarization. Proceedings of International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006. IEEE Computer Society, 2006. pp. 72-75 (Proceedings of International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006).

@inproceedings{a750ac3732a246f89d93121665f4ff10,

title = "Technique for false image correction in second harmonic generation microscopy by modulating laser polarization",

abstract = "Second harmonic generation microscopy (SHGM) has been widely used in biomedicine as a promising non-invasive imaging technique. However, due to the complexity of biological samples, false images are often produced depending on sample orientation relative to laser polarization and on sample parameter related to the second-order optical nonlinear susceptibility. In this work, we present a convenient SHG technique to correct the false images by modulating laser polarization and stitching complementary images together, the stitched image will contain more detailed information and will better represent the sample structures. We first describe our SHG technique with a brief mathematical model, and then show its validity by performing it on two kind of samples: symmetrically synthesized bio-origin polymer poly(3-hyroxybutyrate-co-3- hydroxyhexanoate) (PHBHHx) and fibrillar collagen of rat-tail tendon.",

author = "L. Jin and L. Gao and L. Huo and W. Yuan and Luo, {Y. H.} and Hot, {A. H.P.} and C. Lint",

note = "Copyright: Copyright 2019 Elsevier B.V., All rights reserved.; International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006 ; Conference date: 16-10-2006 Through 18-10-2006",

year = "2006",

doi = "10.1109/METAMAT.2006.335001",

language = "English (US)",

isbn = "0780397746",

series = "Proceedings of International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006",

publisher = "IEEE Computer Society",

pages = "72--75",

booktitle = "Proceedings of International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006",

}

TY - GEN

T1 - Technique for false image correction in second harmonic generation microscopy by modulating laser polarization

AU - Jin, L.

AU - Gao, L.

AU - Huo, L.

AU - Yuan, W.

AU - Luo, Y. H.

AU - Hot, A. H.P.

AU - Lint, C.

PY - 2006

Y1 - 2006

N2 - Second harmonic generation microscopy (SHGM) has been widely used in biomedicine as a promising non-invasive imaging technique. However, due to the complexity of biological samples, false images are often produced depending on sample orientation relative to laser polarization and on sample parameter related to the second-order optical nonlinear susceptibility. In this work, we present a convenient SHG technique to correct the false images by modulating laser polarization and stitching complementary images together, the stitched image will contain more detailed information and will better represent the sample structures. We first describe our SHG technique with a brief mathematical model, and then show its validity by performing it on two kind of samples: symmetrically synthesized bio-origin polymer poly(3-hyroxybutyrate-co-3- hydroxyhexanoate) (PHBHHx) and fibrillar collagen of rat-tail tendon.

AB - Second harmonic generation microscopy (SHGM) has been widely used in biomedicine as a promising non-invasive imaging technique. However, due to the complexity of biological samples, false images are often produced depending on sample orientation relative to laser polarization and on sample parameter related to the second-order optical nonlinear susceptibility. In this work, we present a convenient SHG technique to correct the false images by modulating laser polarization and stitching complementary images together, the stitched image will contain more detailed information and will better represent the sample structures. We first describe our SHG technique with a brief mathematical model, and then show its validity by performing it on two kind of samples: symmetrically synthesized bio-origin polymer poly(3-hyroxybutyrate-co-3- hydroxyhexanoate) (PHBHHx) and fibrillar collagen of rat-tail tendon.

UR - http://www.scopus.com/inward/record.url?scp=46149107089&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=46149107089&partnerID=8YFLogxK

U2 - 10.1109/METAMAT.2006.335001

DO - 10.1109/METAMAT.2006.335001

M3 - Conference contribution

AN - SCOPUS:46149107089

SN - 0780397746

SN - 9780780397743

T3 - Proceedings of International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006

SP - 72

EP - 75

BT - Proceedings of International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006

PB - IEEE Computer Society

T2 - International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006

Y2 - 16 October 2006 through 18 October 2006

ER -

Technique for false image correction in second harmonic generation microscopy by modulating laser polarization

Abstract

Publication series

Other

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this