Tbx3 and Nr5α2 Play Important Roles in Pig Pluripotent Stem Cells

Jianyu Wang, Qi Gu, Jie Hao, Yundan Jia, Binghua Xue, Honghong Jin, Jing Ma, Renyue Wei, Tang Hai, Qingran Kong, Gerelchimeg Bou, Ping Xia, Qi Zhou, Liu Wang, Zhonghua Liu

Research output: Contribution to journalArticle

Abstract

Pigs are valuable animal models in pre-clinical research due to their anatomical and similarity to human-beings. Little is known about porcine embryonic development and porcine pluripotent stem cells. Recently, porcine-induced pluripotent stem cells (piPSCs) have been generated with Oct4 (Pou5f1), Sox2, Klf4 and c-Myc (termed OSKM, 4 F). Here, we found two other factors (Tbx3 and Nr5α2, termed TN), with important roles in piPSCs induction. They could improve the generation of piPSCs by supplementing these two factors on the basis of OSKM (OSKMTN, 6 F) orientated to mouse ESCs-like. Surprisingly, Nr5α2 alone could induce piPSCs formation in the presence or absence of c-Myc. These results suggested that Tbx3 and Nr5α2 may have vital roles in Sus scrofa and proposed new insights into pig pluripotent stem cells.

Original languageEnglish (US)
Pages (from-to)700-708
Number of pages9
JournalStem Cell Reviews and Reports
Volume9
Issue number5
DOIs
StatePublished - Oct 1 2013

Keywords

  • Efficiency
  • Nr5α2
  • Pluripotency
  • Tbx3
  • piPSCs

ASJC Scopus subject areas

  • Cell Biology
  • Cancer Research

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  • Cite this

    Wang, J., Gu, Q., Hao, J., Jia, Y., Xue, B., Jin, H., Ma, J., Wei, R., Hai, T., Kong, Q., Bou, G., Xia, P., Zhou, Q., Wang, L., & Liu, Z. (2013). Tbx3 and Nr5α2 Play Important Roles in Pig Pluripotent Stem Cells. Stem Cell Reviews and Reports, 9(5), 700-708. https://doi.org/10.1007/s12015-013-9439-2